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Factor VII ELISA Kit
F7
Reactivity: Human
Colorimetric
Sandwich ELISA
0.4-100 ng/mL
Cell Culture Supernatant, Plasma, Serum
Product Details Factor VII ELISA Kit
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Target
See all Factor VII (F7) ELISA Kits
Factor VII (F7)
(Coagulation Factor VII (F7))
Reactivity
All reactivities for Factor VII elisa kits
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Detection Range
0.4-100 ng/mL
Minimum Detection Limit
0.4 ng/mL
Application
ELISA
Purpose
Human Coagulation Factor VII ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
Sample Type
Cell Culture Supernatant, Plasma, Serum
Analytical Method
Quantitative
Specificity
The antibody pair provided in this kit recognizes Human Coagulation Factor VII (F7)
Sensitivity
0.4 ng/mL
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Application Details
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Application Notes
Recommended Dilution for serum and plasma samples2 fold
Sample Volume
100 μL
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for Factor VII
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Target
See all Factor VII (F7) ELISA Kits
Factor VII (F7)
(Coagulation Factor VII (F7))
Alternative Name
Coagulation Factor VII (F7 Products )
Synonyms
F7 ELISA Kit, wu:fb59f04 ELISA Kit, zgc:109870 ELISA Kit, SPCA ELISA Kit, AI114908 ELISA Kit, ATIII ELISA Kit, At-3 ELISA Kit, At3 ELISA Kit, LOC100223776 ELISA Kit, AI132620 ELISA Kit, Cf7 ELISA Kit, FVII ELISA Kit, mfVII ELISA Kit, f7 ELISA Kit, coagulation factor VII ELISA Kit, serine (or cysteine) peptidase inhibitor, clade C (antithrombin), member 1 ELISA Kit, coagulation factor 7 (serum prothrombin conversion accelerator) S homeolog ELISA Kit, F7 ELISA Kit, f7 ELISA Kit, Serpinc1 ELISA Kit, CpipJ_CPIJ009142 ELISA Kit, CpipJ_CPIJ010295 ELISA Kit, CpipJ_CPIJ020127 ELISA Kit, fa7 ELISA Kit, f7.S ELISA Kit
Background
Gene Names: F7 Protein names: Coagulation factor VII (EC 3.4.21.21) (Proconvertin) (Serum prothrombin conversion accelerator) (SPCA) (Eptacog alfa) [Cleaved into: Factor VII light chain, Factor VII heavy chain]
Gene ID
2155
UniProt
P08709
Pathways
Response to Growth Hormone Stimulus , Platelet-derived growth Factor Receptor Signaling
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