Fibronectin ELISA Kit
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- Target See all Fibronectin ELISA Kits
- Fibronectin
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Reactivity
- Cow
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 1.2-300 ng/mL
- Minimum Detection Limit
- 1.2 ng/mL
- Application
- ELISA
- Purpose
- Bovine Fibronectin ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
- Sample Type
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- Specificity
- The antibody pair provided in this kit recognizes Bovine Fibronectin (FN1)
- Sensitivity
- 1.2 ng/mL
- Characteristics
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Components
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Material not included
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Application Notes
- Recommended Dilution for serum and plasma samples500 fold
- Sample Volume
- 100 μL
- Plate
- Pre-coated
- Protocol
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Storage
- -20 °C
- Storage Comment
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Expiry Date
- 6 months
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- Target See all Fibronectin ELISA Kits
- Fibronectin
- Abstract
- Fibronectin Products
- Synonyms
- FN ELISA Kit, cig ELISA Kit, fibronectin ELISA Kit, finc ELISA Kit, lets ELISA Kit, msf ELISA Kit, CIG ELISA Kit, ED-B ELISA Kit, FINC ELISA Kit, FNZ ELISA Kit, GFND ELISA Kit, GFND2 ELISA Kit, LETS ELISA Kit, MSF ELISA Kit, E330027I09 ELISA Kit, Fn ELISA Kit, Fn-1 ELISA Kit, FIBNEC ELISA Kit, fn-1 ELISA Kit, fibronectin 1 ELISA Kit, FN1 ELISA Kit, fn1 ELISA Kit, Fn1 ELISA Kit
- Background
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Gene Names: FN1
Protein names: Fibronectin (FN) [Cleaved into: Anastellin]
- UniProt
- P07589
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