OLR1 ELISA Kit

Catalog No. ABIN2703304

Product Details OLR1 ELISA Kit

Target: OLR1 (Oxidized Low Density Lipoprotein (Lectin-Like) Receptor 1 (OLR1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 2-500 pg/mL

Minimum Detection Limit: 2 pg/mL

Application: ELISA

Purpose: Human LOX-1 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Sample Type: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificity: This ELISA antibody pair detects human LOX-1. Other species not determined yet.

Sensitivity: 2 pg/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples2 - 10 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for OLR1

Target: OLR1 (Oxidized Low Density Lipoprotein (Lectin-Like) Receptor 1 (OLR1))

Alternative Name: LOX-1 (OLR1 Products)

Synonyms: OLR1 ELISA Kit, LOX1 ELISA Kit, Olr1 ELISA Kit, CLEC8A ELISA Kit, LOXIN ELISA Kit, SCARE1 ELISA Kit, SLOX1 ELISA Kit, LOX-1 ELISA Kit, SR-EI ELISA Kit, Scare1 ELISA Kit, Oldlr1 ELISA Kit, Oldr1 ELISA Kit, PLOX-1 ELISA Kit, oxidized low density lipoprotein receptor 1 ELISA Kit, oxidized low density lipoprotein (lectin-like) receptor 1 ELISA Kit, OLR1 ELISA Kit, Olr1 ELISA Kit

Background: Gene Names: OLR1 CLEC8A LOX1
Protein names: Oxidized low-density lipoprotein receptor 1 (Ox-LDL receptor 1) (C-type lectin domain family 8 member A) (Lectin-like oxidized LDL receptor 1) (LOX-1) (Lectin-like oxLDL receptor 1) (hLOX-1) (Lectin-type oxidized LDL receptor 1) [Cleaved into: Oxidized low-density lipoprotein receptor 1, soluble form]

Gene ID: 4973

UniProt: P78380