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PAPPA ELISA Kit
PAPPA
Reactivity: Human
Colorimetric
Sandwich ELISA
0.6-500 ng/mL
Cell Culture Supernatant, Plasma, Serum
Product Details PAPPA ELISA Kit
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Target
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PAPPA
(Pregnancy-Associated Plasma Protein A, Pappalysin 1 (PAPPA))
Reactivity
All reactivities for PAPPA elisa kits
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Detection Range
0.6-500 ng/mL
Minimum Detection Limit
0.6 ng/mL
Application
ELISA
Purpose
Human PAPP-A ELISA Kit for cell culture supernatants, plasma, and serum samples.
Sample Type
Cell Culture Supernatant, Plasma, Serum
Analytical Method
Quantitative
Specificity
Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human Alpha-2 Microglobulin, ApoA, ApoC1, ApoC2, ApoC3, ApoE, ApoH, Artemin, BMP-6, Calbindin, CK beta 8-1 (CCL23), CNTF, EMMPRIN (CD147), Endostatin, Fibrinogen, FLRG, TNFSF18 (GITR Ligand), GPBB, Hemoglobin, HTRA2, IL-17E, IL-1 R3 (IL1 R Acp), Lactoferrin, Mesothelin, PAPP-A, PD-ECGF (Thymidine Phosphorylase), PDGF R alpha, Pepsinogen 2, Periostin, Persephin, PTH, RAP, SAA (Serum Amyloid A), Serpin F1 (PEDF), Tie-1, TPA, Thrombospondin 2, Ubiquitin+1, Vitronectin, VWF
Sensitivity
0.6 ng/mL
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Application Details
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Application Notes
Recommended Dilution for serum and plasma samples2 fold
Sample Volume
100 μL
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for PAPPA
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Target
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PAPPA
(Pregnancy-Associated Plasma Protein A, Pappalysin 1 (PAPPA))
Alternative Name
PAPP-A (PAPPA Products )
Synonyms
8430414N03Rik ELISA Kit, IGFBP-4ase ELISA Kit, PAG1 ELISA Kit, PAPP-A ELISA Kit, ASBABP2 ELISA Kit, DIPLA1 ELISA Kit, PAPA ELISA Kit, PAPPA1 ELISA Kit, Pappa ELISA Kit, pappalysin 1 ELISA Kit, pregnancy-associated plasma protein A ELISA Kit, PAPPA ELISA Kit, Pappa ELISA Kit, Pappa1 ELISA Kit
Background
Gene Names: PAPPA Protein names: Pappalysin-1 (EC 3.4.24.79) (Insulin-like growth factor-dependent IGF-binding protein 4 protease) (IGF-dependent IGFBP-4 protease) (IGFBP-4ase) (Pregnancy-associated plasma protein A) (PAPP-A)
Gene ID
5069
UniProt
Q13219
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