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SERPINA4 ELISA Kit
SERPINA4
Reactivity: Human
Colorimetric
Sandwich ELISA
0.12-35 ng/mL
Cell Culture Supernatant, Plasma, Serum
Product Details SERPINA4 ELISA Kit
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Target
See all SERPINA4 ELISA Kits
SERPINA4
(serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 4 (SERPINA4))
Reactivity
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Detection Range
0.12-35 ng/mL
Minimum Detection Limit
0.12 ng/mL
Application
ELISA
Purpose
Human SERPINA4 ELISA Kit for cell culture supernatants, plasma, and serum samples.
Sample Type
Plasma, Cell Culture Supernatant, Serum
Analytical Method
Quantitative
Specificity
This ELISA antibody pair detects human Serpin A4. Other species not determined.
Sensitivity
0.12 ng/mL
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Application Details
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Application Notes
Recommended Dilution for serum and plasma samples10,000 fold
Sample Volume
100 μL
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for SERPINA4
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Target
See all SERPINA4 ELISA Kits
SERPINA4
(serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 4 (SERPINA4))
Alternative Name
Serpin A4 (SERPINA4 Products )
Synonyms
KLKR ELISA Kit, Klk6 ELISA Kit, hK1 ELISA Kit, KAL ELISA Kit, KLST ELISA Kit, KST ELISA Kit, PI-4 ELISA Kit, PI4 ELISA Kit, kallistatin ELISA Kit, SERPINA4 ELISA Kit, DKFZp469E1737 ELISA Kit, KALps ELISA Kit, Klst ELISA Kit, kallikrein 1 ELISA Kit, serpin family A member 4 ELISA Kit, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 4 ELISA Kit, serine (or cysteine) peptidase inhibitor, clade A, member 4, pseudogene 1 ELISA Kit, KLK1 ELISA Kit, SERPINA4 ELISA Kit, Serpina4 ELISA Kit, SPIA4 ELISA Kit, Serpina4-ps1 ELISA Kit
Background
Gene Names: SERPINA4 KST PI4 Protein names: Kallistatin (Kallikrein inhibitor) (Peptidase inhibitor 4) (PI-4) (Serpin A4)
Gene ID
5267
UniProt
P29622
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