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SERPING1 ELISA Kit
SERPING1
Reactivity: Human
Colorimetric
Sandwich ELISA
2.4-600 ng/mL
Cell Culture Supernatant, Plasma, Serum
Product Details SERPING1 ELISA Kit
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Target
See all SERPING1 ELISA Kits
SERPING1
(Serpin Peptidase Inhibitor, Clade G (C1 Inhibitor), Member 1 (SERPING1))
Reactivity
All reactivities for SERPING1 elisa kits
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Detection Range
2.4-600 ng/mL
Minimum Detection Limit
2.4 ng/mL
Application
ELISA
Purpose
Human SERPING1 ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
Sample Type
Cell Culture Supernatant, Plasma, Serum
Analytical Method
Quantitative
Specificity
This ELISA antibody pair recognizes human SERPING1. Other species not yet determined.
Sensitivity
2.4 ng/mL
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Application Details
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Application Notes
Recommended Dilution for serum and plasma samples600 fold
Sample Volume
100 μL
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for SERPING1
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Target
See all SERPING1 ELISA Kits
SERPING1
(Serpin Peptidase Inhibitor, Clade G (C1 Inhibitor), Member 1 (SERPING1))
Alternative Name
Serpin G1 (SERPING1 Products )
Synonyms
fb64f02 ELISA Kit, im:7159527 ELISA Kit, si:dkeyp-110g5.2 ELISA Kit, wu:fb60c09 ELISA Kit, wu:fb62a08 ELISA Kit, wu:fb64f02 ELISA Kit, LOC100136072 ELISA Kit, C1-INH ELISA Kit, C1INH ELISA Kit, C1nh ELISA Kit, C1IN ELISA Kit, C1NH ELISA Kit, HAE1 ELISA Kit, HAE2 ELISA Kit, serpin peptidase inhibitor, clade G (C1 inhibitor), member 1 ELISA Kit, C1 inhibitor ELISA Kit, serpin family G member 1 ELISA Kit, serine (or cysteine) peptidase inhibitor, clade G, member 1 ELISA Kit, serping1 ELISA Kit, LOC100136072 ELISA Kit, SERPING1 ELISA Kit, Serping1 ELISA Kit
Background
Gene Names: SERPING1 C1IN C1NH Protein names: Plasma protease C1 inhibitor (C1 Inh) (C1Inh) (C1 esterase inhibitor) (C1-inhibiting factor) (Serpin G1)
Gene ID
710
UniProt
P05155
Pathways
Complement System
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