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TGFBR3 ELISA Kit
TGFBR3
Reactivity: Human
Colorimetric
Sandwich ELISA
40-10.000 pg/mL
Cell Culture Supernatant, Plasma, Serum
Product Details TGFBR3 ELISA Kit
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Target
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TGFBR3
(Transforming Growth Factor, beta Receptor III (TGFBR3))
Reactivity
All reactivities for TGFBR3 elisa kits
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Detection Range
40-10.000 pg/mL
Minimum Detection Limit
40 pg/mL
Application
ELISA
Purpose
Human TGF beta R3 ELISA Kit for cell culture supernatants, plasma, and serum samples.
Sample Type
Cell Culture Supernatant, Plasma, Serum
Analytical Method
Quantitative
Specificity
This ELISA antibody pair shows no cross-reactivity with recombinant human (rh) TGF-beta RI, RII, RIIb, TGF-beta 1, beta 2, beta 3, recombinant mouse TGF-beta RIII.
Sensitivity
40 pg/mL
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Application Details
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Application Notes
Recommended Dilution for serum and plasma samples20 fold
Sample Volume
100 μL
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for TGFBR3
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Target
See all TGFBR3 ELISA Kits
TGFBR3
(Transforming Growth Factor, beta Receptor III (TGFBR3))
Alternative Name
TGF-beta R3 (TGFBR3 Products )
Synonyms
TGFBR3 ELISA Kit, DKFZp469I0915 ELISA Kit, BGCAN ELISA Kit, betaglycan ELISA Kit, 1110036H20Rik ELISA Kit, AU015626 ELISA Kit, AW215636 ELISA Kit, TBRIII ELISA Kit, transforming growth factor beta receptor 3 ELISA Kit, transforming growth factor, beta receptor III ELISA Kit, TGFBR3 ELISA Kit, tgfbr3 ELISA Kit, Tgfbr3 ELISA Kit
Background
Gene Names: TGFBR3 Protein names: Transforming growth factor beta receptor type 3 (TGF-beta receptor type 3) (TGFR-3) (Betaglycan) (Transforming growth factor beta receptor III) (TGF-beta receptor type III)
Gene ID
7049
UniProt
Q03167
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