CD137 ELISA Kit
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- Target See all CD137 (TNFRSF9) ELISA Kits
- CD137 (TNFRSF9) (Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9))
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Reactivity
- Rat
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Application
- ELISA
- Purpose
- Custom Rat 4-1BB (TNFRSF9/CD137) ELISA Kit.
- Sample Type
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- Specificity
- The antibody pair provided in this kit recognizes Rat 4-1BB (TNFRSF9/CD137)
- Characteristics
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Components
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Material not included
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Sample Volume
- 100 μL
- Plate
- Pre-coated
- Protocol
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Assay Procedure
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Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Storage
- -20 °C
- Storage Comment
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Expiry Date
- 6 months
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- Target See all CD137 (TNFRSF9) ELISA Kits
- CD137 (TNFRSF9) (Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9))
- Alternative Name
- 4-1BB (TNFRSF9 Products)
- Synonyms
- TNFRSF9 ELISA Kit, zgc:136557 ELISA Kit, 4-1BB ELISA Kit, CD137 ELISA Kit, CDw137 ELISA Kit, ILA ELISA Kit, A930040I11Rik ELISA Kit, AA408498 ELISA Kit, AI325004 ELISA Kit, Cd137 ELISA Kit, Ly63 ELISA Kit, tumor necrosis factor receptor superfamily member 9 ELISA Kit, TNF receptor superfamily member 9 ELISA Kit, tumor necrosis factor receptor superfamily, member 9a ELISA Kit, tumor necrosis factor receptor superfamily, member 9 ELISA Kit, TNFRSF9 ELISA Kit, tnfrsf9a ELISA Kit, Tnfrsf9 ELISA Kit
- Background
- 4-1BB (TNFRSF9/CD137)
- UniProt
- Q4W8J3
- Pathways
- Cancer Immune Checkpoints
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