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MSK2 ELISA Kit

RPS6KA4 Reactivity: Human pSer360 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Catalog No. ABIN2748386
  • Target See all MSK2 (RPS6KA4) ELISA Kits
    MSK2 (RPS6KA4) (Ribosomal Protein S6 Kinase A4 (RPS6KA4))
    Binding Specificity
    pSer360, total
    Reactivity
    • 3
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Human Phospho-MSK2 (S360) & Total MSK2 ELISA Kit. This assay semi-quantitatively measures phophorylated MSK2 (Ser360) & Total MSK2 in lysate samples.
    Sample Type
    Cell Lysate, Tissue Lysate
    Analytical Method
    Semi-Quantitative
    Specificity
    The antibody pair provided in this kit recognizes human MSK2 phosphorylated at site Serine-360.
    Characteristics
    • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • Anti-Pan Antibody
    • HRP-Conjugated Secondary Antibody
    • Streptavidin-Conjugated HRP
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Material not included
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
    Top Product
    Discover our top product RPS6KA4 ELISA Kit
  • Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Assay Procedure

    Prepare all reagents and samples as instructed in the manual.
    Add 100 μL of sample or positive control to each well.
    Incubate 2.5 h at RT or O/N at 4 °C.
    Add 100 μL of prepared primary antibody to each well.
    Incubate 1 h at RT.
    Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    Incubate 1 h at RT.
    Add 100 μL of TMB One-Step Substrate Reagent to each well.
    Incubate 30 min at RT.
    Add 50 μL of Stop Solution to each well.
    Read at 450 nm immediately.

    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Expiry Date
    6 months
  • Target See all MSK2 (RPS6KA4) ELISA Kits
    MSK2 (RPS6KA4) (Ribosomal Protein S6 Kinase A4 (RPS6KA4))
    Alternative Name
    MSK2 (RPS6KA4 Products)
    Synonyms
    MSK2 ELISA Kit, RSK-B ELISA Kit, 1110069D02Rik ELISA Kit, 90kDa ELISA Kit, AI848992 ELISA Kit, Msk2 ELISA Kit, mMSK2 ELISA Kit, ribosomal protein S6 kinase A4 ELISA Kit, ribosomal protein S6 kinase, polypeptide 4 ELISA Kit, RPS6KA4 ELISA Kit, Rps6ka4 ELISA Kit
    Background
    Mitogen- and stress-activated protein kinase 2 (MSK2) phosphorylated at Serine-360 and Total MSK2
    Gene ID
    8986
    UniProt
    O75676
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