TFPI ELISA Kit

Catalog No. ABIN2859226

Product Details TFPI ELISA Kit

Target: TFPI (Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI))

Binding Specificity: AA 29-306

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156-10.000 pg/mL

Minimum Detection Limit: 156 pg/mL

Application: ELISA

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse TFPI

Brand: PicoKine™

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO
Immunogen sequence: L29-N306

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: L29-N306

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Tissue Specificity: Isoform alpha is expressed in heart and spleen, isoform beta in heart and lung.

Plate: Pre-coated

Protocol: mouse TFPI ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TFPI has been precoated onto 96-well plates. Standards(NSO, L29-N306) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TFPI is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TFPI amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse TFPI standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse TFPI standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(ng/ml): 1.05, Standard deviation: 0.048, CV(%): 4.6
• Sample 2: n=16, Mean(ng/ml): 2.91, Standard deviation: 0.122, CV(%): 4.2
• Sample 3: n=16, Mean(ng/ml): 5.34, Standard deviation: 0.203, CV(%): 3.8,
• Sample 1: n=24, Mean(ng/ml): 1.21, Standard deviation: 0.076, CV(%): 6.3
• Sample 2: n=24, Mean(ng/ml): 3.45, Standard deviation: 0.193, CV(%): 5.6
• Sample 3: n=24, Mean(ng/ml): 5.82, Standard deviation: 0.285, CV(%): 4.9

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

Target Details for TFPI

Target: TFPI (Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI))

Alternative Name: TFPI (TFPI Products)

Synonyms: EPI ELISA Kit, LACI ELISA Kit, TFI ELISA Kit, TFPI1 ELISA Kit, A630013F22Rik ELISA Kit, AW552122 ELISA Kit, TFPI ELISA Kit, si:dz44f8.1 ELISA Kit, tfpib ELISA Kit, wu:fb92g01 ELISA Kit, zgc:152844 ELISA Kit, zgc:158592 ELISA Kit, tissue factor pathway inhibitor ELISA Kit, tissue factor pathway inhibitor a ELISA Kit, TFPI ELISA Kit, Tfpi ELISA Kit, tfpia ELISA Kit

Background:

Protein Function: Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma (By similarity). .

Background: TFPI(Tissue factor pathway inhibitor),also known as EPI, LACI or TFPI1, is a single-chain polypeptide which can reversibly inhibit Factor Xa (Xa) and Thrombin (Factor IIa). The gene for TFPI is located on chromosome 2q31-q32.1, and has nine exons which span 70 kb. TFPI consists of a highly negatively-charged amino-terminus, three tandemly-linked Kunitz domains, and a highly positively-charged carboxy-terminus. TFPI contributes significantly to the inhibition of Xa in vivo, despite being present at concentrations of only 2.5 nM.

Synonyms: Tissue factor pathway inhibitor,TFPI,Extrinsic pathway inhibitor,EPI,Lipoprotein-associated coagulation inhibitor,LACI,Tfpi,

Full Gene Name: Tissue factor pathway inhibitor

Cellular Localisation: Secreted.

Gene ID: 21788

UniProt: O54819