Glucagon FLISA Kit
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- Target See all Glucagon (GCG) FLISA Kits
- Glucagon (GCG)
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Reactivity
- Human, Mouse, Rat, Pig, Cow
- Detection Method
- Fluorometric
- Method Type
- Competition ELISA
- Application
- ELISA
- Analytical Method
- Quantitative
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- Discover our best selling GCG ELISA Kit
- Top Product
- Discover our top product GCG ELISA Kit
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Plate
- Pre-coated
- Protocol
- The immunoplate in this kit is pre-coated with a secondary antibody and the nonspecific binding sites are blocked. The secondary antibody can bind to the Fc fragment of the primary antibody (peptide antibody) whose Fab fragment will be competitively bound by both biotinylated peptide and peptide standard or targeted peptide in samples. The biotinylated peptide interacts with streptavidin-horseradish peroxidase (SA-HRP) which catalyzes the substrate. The fluorescence intensity is directly proportional to the amount of biotinylated peptide-SA-HRP complex but inversely proportional to the amount of the peptide in standard solutions or samples. This is due to the competitive binding of the biotinylated peptide with the standard peptide or samples to the peptide antibody (primary antibody). A standard curve of known concentration can be established accordingly. The unknown concentration in samples can be determined by extrapolation to this standard curve.
- Restrictions
- For Research Use only
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- Storage
- 4 °C
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- Target See all Glucagon (GCG) FLISA Kits
- Glucagon (GCG)
- Alternative Name
- Glucagon (GCG Products)
- Synonyms
- GLP1 FLISA Kit, GLP2 FLISA Kit, GRPP FLISA Kit, GLP-1 FLISA Kit, Glu FLISA Kit, PPG FLISA Kit, GCG FLISA Kit, gcg-A FLISA Kit, gcg1 FLISA Kit, glucagon FLISA Kit, glucagon L homeolog FLISA Kit, GCG FLISA Kit, Gcg FLISA Kit, gcg.L FLISA Kit
- Pathways
- Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, cAMP Metabolic Process, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Negative Regulation of intrinsic apoptotic Signaling
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