TGFB1 ELISA Kit

Catalog No. ABIN411354

The Rat TGFB1 ELISA Kit (ABIN411354) is a Colorimetric ELISA Kit designed to quantify Rat TGFB1. This ELISA Kit has been cited in 23 publications.

Quick Overview for TGFB1 ELISA Kit (ABIN411354)

Target: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Binding Specificity: AA 279-390

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6 pg/mL - 1000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (EDTA), Urine

Product Details TGFB1 ELISA Kit

Minimum Detection Limit: 15.6 pg/mL

Purpose: Sandwich Rat TGF Beta 1 ELISA Kit to quantitate Rat Tgfb1 in cell culture supernatants, serum, plasma (EDTA) and urine.

Analytical Method: Quantitative

Specificity: Expression system for standard: CHO
Immunogen sequence: A279-S390

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Cross-Reactivity (Details): There is cross-reactivity with TGFbeta2, TGFbeta3, TGFbeta5<1 % .

Sensitivity: < 1 pg/mL

Components:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Assay Precision: Intra-Assay Precision (CV%): 5.5%, 6.1%, 5.5%
Inter-Assay Precision (CV%): 6.2%, 6.4%, 6.2%

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Expiry Date: 12 months

References for TGFB1 ELISA Kit (ABIN411354)

Tian, Tian, Huo, Chang, Zheng, Du, Chen, Niu: "Bacillus Calmette-Guerin alleviates airway inflammation and remodeling by preventing TGF-β1 induced epithelial-mesenchymal transition." in: Human vaccines & immunotherapeutics, Vol. 13, Issue 8, pp. 1758-1764, (2018) (PubMed).

Luo, Liu, Zhao, He, Li, Zha, Li, Qi, Liu, Yu: "Galectin-3 mediates pulmonary vascular remodeling in hypoxia-induced pulmonary arterial hypertension." in: Journal of the American Society of Hypertension : JASH, Vol. 11, Issue 10, pp. 673-683.e3, (2018) (PubMed).

Sarandy, Novaes, Xavier, Vital, Leite, Melo, Gonçalves: "Hydroethanolic Extract of Strychnos pseudoquina Accelerates Skin Wound Healing by Modulating the Oxidative Status and Microstructural Reorganization of Scar Tissue in Experimental Type I Diabetes." in: BioMed research international, Vol. 2017, pp. 9538351, (2018) (PubMed).

Sarandy, Miranda, Altoé, Novaes, Zanuncio, Leite, Gonçalves: "Strychnos pseudoquina modulates the morphological reorganization of the scar tissue of second intention cutaneous wounds in rats." in: PLoS ONE, Vol. 13, Issue 4, pp. e0195786, (2018) (PubMed).

Fu, Wang, Cai, Zhao, Fu: "Theaflavin alleviates inflammatory response and brain injury induced by cerebral hemorrhage via inhibiting the nuclear transcription factor kappa β-related pathway in rats." in: Drug design, development and therapy, Vol. 12, pp. 1609-1619, (2018) (PubMed).

Xiang, Han, Zhang, Yan, Xu, Chu, Xie, Jia, Yan, Zhao, Wang, Lei: "A New Oleanolic Acid Derivative against CCl₄-Induced Hepatic Fibrosis in Rats." in: International journal of molecular sciences, Vol. 18, Issue 3, (2017) (PubMed).

Zhang, Li, Li, Liu, Guo, Xu: "Nitric Oxide Synthase Activity Correlates with OGG1 in Ozone-Induced Lung Injury Animal Models." in: Frontiers in physiology, Vol. 8, pp. 249, (2017) (PubMed).

Yu, Mi, Long: "Efficacies of rosiglitazone and retinoin on bleomycin-induced pulmonary fibrosis in rats." in: Experimental and therapeutic medicine, Vol. 14, Issue 1, pp. 609-615, (2017) (PubMed).

Zhang, Li, Huang, Wang: "Danggui buxue tang suppresses high glucose-induced proliferation and extracellular matrix accumulation of mesangial cells via inhibiting lncRNA PVT1." in: American journal of translational research, Vol. 9, Issue 8, pp. 3732-3740, (2017) (PubMed).

Liao, Li, Yuan, Chen, Zuo, Rashid, Zhang, Feng, Liu: "LSKL peptide alleviates subarachnoid fibrosis and hydrocephalus by inhibiting TSP1-mediated TGF-β1 signaling activity following subarachnoid hemorrhage in rats." in: Experimental and therapeutic medicine, Vol. 12, Issue 4, pp. 2537-2543, (2016) (PubMed).

Liu, Fan, Chen: "3,4-dihydroxyphenylethanol suppresses irradiation-induced pulmonary fibrosis in adult rats." in: International journal of clinical and experimental pathology, Vol. 8, Issue 4, pp. 3441-50, (2015) (PubMed).

Hou, Zhu, Pang, Jeffry, Zhou: "Protective effect of iridoid glycosides from Paederia scandens (LOUR.) MERRILL (Rubiaceae) on uric acid nephropathy rats induced by yeast and potassium oxonate." in: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, Vol. 64, pp. 57-64, (2014) (PubMed).

Sharifi, Amani, Hajiani, Cheraghian: "Does vitamin D improve liver enzymes, oxidative stress, and inflammatory biomarkers in adults with non-alcoholic fatty liver disease? A randomized clinical trial." in: Endocrine, Vol. 47, Issue 1, pp. 70-80, (2014) (PubMed).

Zhai, Lu, Liu, Cheng, Wei, Zhang, Li, Yin: "Over-production of nitric oxide by oxidative stress-induced activation of the TGF-β1/PI3K/Akt pathway in mesangial cells cultured in high glucose." in: Acta pharmacologica Sinica, Vol. 34, Issue 4, pp. 507-14, (2014) (PubMed).

Abo-Gresha, Abel-Aziz, Greish: "Evening primrose oil ameliorates platelet aggregation and improves cardiac recovery in myocardial-infarct hypercholesterolemic rats." in: International journal of physiology, pathophysiology and pharmacology, Vol. 6, Issue 1, pp. 23-36, (2014) (PubMed).

You, Wei, Sun, Wang, Yang, Liu, Zheng, Wang, Zhang: "The green tea extract epigallocatechin-3-gallate inhibits irradiation-induced pulmonary fibrosis in adult rats." in: International journal of molecular medicine, Vol. 34, Issue 1, pp. 92-102, (2014) (PubMed).

Wenbin, Guojun: "Resveratrol Ameliorates Diabetes-induced Renal Damage through Regulating the Expression of TGF-?1, Collagen IV and Th17/Treg-related Cytokines in Rats." in: The West Indian medical journal, Vol. 63, Issue 1, pp. 20-5, (2014) (PubMed).

Gao, Huang, Li: "NOX1 abet mesangial fibrogenesis via iNOS induction in diabetes." in: Molecular and cellular biochemistry, Vol. 382, Issue 1-2, pp. 185-91, (2013) (PubMed).

Li, Tian: "Correlation between apoptosis and TGF-β1 expression in the mucosal epithelium of rat small intestine in a cold stress state." in: Experimental and therapeutic medicine, Vol. 5, Issue 5, pp. 1456-1460, (2013) (PubMed).

He, Qi, Rong, Jiang, Yang, Yamahara, Murray, Li: "The Ayurvedic Medicine Salacia oblonga Attenuates Diabetic Renal Fibrosis in Rats: Suppression of Angiotensin II/AT1 Signaling." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2011, pp. 807451, (2012) (PubMed).

Target Details for TGFB1

Target: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Alternative Name: TGFB1

Background:

Background: Transforming growth factor-beta1 (TGF-beta1) is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. Many cells synthesize TGF-beta and essentially all of them have specific receptors for this peptide. TGF-beta regulates the actions of many other peptide growth factors and determines a positive or negative ion of their effects. TGFbeta1 is known for its potent and diverse biological effects, including immune regulation, and cell growth and differentiation. TGFbeta1 is also an important mediator of bone remodeling. TGFbeta1, a potent keratinocyte growth inhibitor, has been shown to be overexpressed in keratinocytes in certain inflammatory skin diseases and has been thought to counteract the effects of other growth factors at the site of inflammation. TGF-beta1, a multifunctional cytokine with fibrogenic properties, has been implicated in the pathogenesis of the vascular and target organ complications of hypertension. TGF-beta1 may also regulate blood pressure via stimulation of endothelin-1 and/or renin secretion. TGFbeta1 is secreted as a latent form, which consists of its mature form and a latency-associated peptide (beta1-LAP) in either the presence or the absence of additional latent TGF-beta1-binding protein. The standard product used in this kit is recombinant TGFbeta1 with the molecular mass of 25KDa.

Gene Full Name: transforming growth factor, beta 1

Gene ID: 59086

UniProt: P17246

Pathways: EGFR Signaling Pathway, Dopaminergic Neurogenesis, Cellular Response to Molecule of Bacterial Origin, Glycosaminoglycan Metabolic Process, Regulation of Leukocyte Mediated Immunity, Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Ribonucleoside Biosynthetic Process, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy, Cancer Immune Checkpoints