TNFSF8 ELISA Kit

Catalog No. ABIN411376

Product Details TNFSF8 ELISA Kit

Target: TNFSF8 (Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8))

Binding Specificity: AA 68-239

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6-1000 pg/mL

Minimum Detection Limit: 15.6 pg/mL

Application: ELISA

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CD30L

Brand: PicoKine™

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO
Immunogen sequence: Q68-D239

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <6pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: Q68-D239

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plate: Pre-coated

Protocol: mouse CD30L ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD30L has been precoated onto 96-well plates. Standards(NSO, Q68-D239) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD30L is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD30L amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL mouse CD30L standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse CD30L standard solution and each sample is measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 92, Standard deviation: 4.51, CV(%): 4.9
• Sample 2: n=16, Mean(pg/ml): 349, Standard deviation: 24.08, CV(%): 6.9
• Sample 3: n=16, Mean(pg/ml): 661, Standard deviation: 40.98, CV(%): 6.2,
• Sample 1: n=24, Mean(pg/ml): 105, Standard deviation: 6.93, CV(%): 5.6
• Sample 2: n=24, Mean(pg/ml): 357, Standard deviation: 25.35, CV(%): 7.1
• Sample 3: n=24, Mean(pg/ml): 711, Standard deviation: 51.90, CV(%): 7.3

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

Target Details for TNFSF8

Target: TNFSF8 (Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8))

Alternative Name: TNFSF8 (TNFSF8 Products)

Synonyms: CD153 ELISA Kit, CD30LG ELISA Kit, Cd30l ELISA Kit, CD30L ELISA Kit, TNFRSF8 ELISA Kit, TNFSF8 ELISA Kit, tumor necrosis factor (ligand) superfamily, member 8 ELISA Kit, TNF superfamily member 8 ELISA Kit, tumor necrosis factor superfamily member 8 ELISA Kit, Tnfsf8 ELISA Kit, TNFSF8 ELISA Kit

Background:

Protein Function: Cytokine that binds to TNFRSF8/CD30. Induces proliferation of T-cells.

Background: The human CD30L(CD153) gene is located at 9q33. It contains 4 exons and spans more than 17.1 kb. CD153 is expressed on the surface of B cells and this expression is upregulated upon CD154(CD40LG), IL4, and B-cell receptor engagement. In these cells, engagement of CD153 by T cell CD30 inhibits immunoglobulin class switch recognition as well as IgG, IgA, and IgE production, suggesting that this"reverse signaling" modulates the CD154-dependent switching of B cells into the pool producing IgG, IgA, and IgE. Additionally, recombinant human CD30L enhanced the proliferation of CD3 -activated T cells, but induced differential responses, including cell death, in several CD30-positive lymphoma-derived cell lines.The standard product used in this kit is recombinant mouse CD30L with the molecular mass of 30-45KDa.

Synonyms: Tumor necrosis factor ligand superfamily member 8,CD30 ligand,CD30-L,CD153,Tnfsf8,Cd30l, Cd30lg,

Full Gene Name: Tumor necrosis factor ligand superfamily member 8

Cellular Localisation: Membrane, Single-pass type II membrane protein.

Gene ID: 21949

UniProt: P32972