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ACVR2B ELISA Kit
ACVR2B
Reactivity: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Product Details ACVR2B ELISA Kit
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Target
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ACVR2B
(Activin A Receptor, Type IIB (ACVR2B))
Reactivity
All reactivities for ACVR2B elisa kits
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Application
ELISA
Purpose
Custom Human Activin R2B (ACVR2B) ELISA Kit.
Sample Type
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificity
This ELISA antibody pair recognizes Human Activin R2B. Other species not yet determined.
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Application Details
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Sample Volume
100 μL
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for ACVR2B
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Target
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ACVR2B
(Activin A Receptor, Type IIB (ACVR2B))
Alternative Name
Activin R2B (ACVR2B Products )
Synonyms
ACVR2B ELISA Kit, XAR1 ELISA Kit, actr-iib ELISA Kit, actriib ELISA Kit, ACTRIIB ELISA Kit, ActR-IIB ELISA Kit, HTX4 ELISA Kit, ActRIIB ELISA Kit, actr2b ELISA Kit, actrIIb ELISA Kit, wu:fj97d11 ELISA Kit, activin A receptor type 2B ELISA Kit, activin A receptor type 2B L homeolog ELISA Kit, activin A receptor type 2Ba ELISA Kit, activin receptor IIB ELISA Kit, ACVR2B ELISA Kit, acvr2b ELISA Kit, acvr2b.L ELISA Kit, Acvr2b ELISA Kit, acvr2ba ELISA Kit
Gene ID
93
UniProt
Q13705
Pathways
Hormone Transport , Cancer Immune Checkpoints
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