CD131 ELISA Kit

Catalog No. ABIN4882498

Product Details CD131 ELISA Kit

Target: CD131 (CSF2RB) (Colony Stimulating Factor 2 Receptor, Beta (CSF2RB))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Custom Human Common beta Chain (CSF2RB) ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: The antibody pair provided in this kit recognizes Human Common beta Chain (CSF2RB)

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for CD131

Target: CD131 (CSF2RB) (Colony Stimulating Factor 2 Receptor, Beta (CSF2RB))

Alternative Name: Common beta Chain (CSF2RB Products)

Synonyms: Csf2rb1 ELISA Kit, CSF2RB ELISA Kit, cd131 ELISA Kit, cdw131 ELISA Kit, il3rb ELISA Kit, il5rb ELISA Kit, il-3rbc ELISA Kit, CD131 ELISA Kit, CDw131 ELISA Kit, IL3RB ELISA Kit, IL5RB ELISA Kit, SMDP5 ELISA Kit, AI848964 ELISA Kit, AIC2B ELISA Kit, Bc ELISA Kit, Csfgmrb ELISA Kit, Il3r ELISA Kit, Il3rb ELISA Kit, Il3rb1 ELISA Kit, Il5rb ELISA Kit, colony stimulating factor 2 receptor beta common subunit ELISA Kit, colony stimulating factor 2 receptor beta common subunit S homeolog ELISA Kit, cytokine receptor common subunit beta-like ELISA Kit, colony stimulating factor 2 receptor, beta, low-affinity (granulocyte-macrophage) ELISA Kit, CSF2RB ELISA Kit, Csf2rb ELISA Kit, csf2rb.S ELISA Kit, CRCBL ELISA Kit, csf2rb ELISA Kit

Gene ID: 1439

UniProt: P32927

Pathways: JAK-STAT Signaling