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KPNA2 ELISA Kit
KPNA2
Reactivity: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Product Details KPNA2 ELISA Kit
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Target
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KPNA2
(Karyopherin alpha 2 (RAG Cohort 1, Importin alpha 1) (KPNA2))
Reactivity
All reactivities for KPNA2 elisa kits
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Application
ELISA
Purpose
Custom Human Importin alpha 2/KPNA2/SRP1-alpha ELISA Kit.
Sample Type
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificity
This ELISA antibody pair recognizes .
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Application Details
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Sample Volume
100 μL
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for KPNA2
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Target
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KPNA2
(Karyopherin alpha 2 (RAG Cohort 1, Importin alpha 1) (KPNA2))
Alternative Name
Importin alpha 2 (KPNA2 Products )
Synonyms
IPOA1 ELISA Kit, QIP2 ELISA Kit, RCH1 ELISA Kit, SRP1alpha ELISA Kit, ima2 ELISA Kit, importin ELISA Kit, 2410044B12Rik ELISA Kit, PTAC58 ELISA Kit, Rch1 ELISA Kit, ipoa1 ELISA Kit, kpna2 ELISA Kit, qip2 ELISA Kit, rch1 ELISA Kit, srp1alpha ELISA Kit, hm:zeh0389 ELISA Kit, hm:zeh0389r ELISA Kit, zgc:113902 ELISA Kit, zgc:86945 ELISA Kit, karyopherin subunit alpha 2 ELISA Kit, importin subunit alpha-2 ELISA Kit, Importin subunit alpha-2 ELISA Kit, karyopherin (importin) alpha 2 ELISA Kit, karyopherin alpha-2 subunit like L homeolog ELISA Kit, karyopherin alpha 2 (RAG cohort 1, importin alpha 1) ELISA Kit, KPNA2 ELISA Kit, EDI_246290 ELISA Kit, EDI_335040 ELISA Kit, ima2 ELISA Kit, Kpna2 ELISA Kit, kpna2.L ELISA Kit, kpna2 ELISA Kit
Gene ID
3838
UniProt
P52292
Pathways
M Phase , Protein targeting to Nucleus
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