PRCP ELISA Kit
-
- Target See all PRCP ELISA Kits
- PRCP (Prolylcarboxypeptidase (PRCP))
-
Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Application
- ELISA
- Purpose
- Custom Human PRCP ELISA Kit.
- Sample Type
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- Characteristics
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Components
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Material not included
-
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Discover our best selling PRCP ELISA Kit
- Top Product
- Discover our top product PRCP ELISA Kit
-
-
- Sample Volume
- 100 μL
- Plate
- Pre-coated
- Protocol
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
-
- Storage
- -20 °C
- Storage Comment
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Expiry Date
- 6 months
-
- Target See all PRCP ELISA Kits
- PRCP (Prolylcarboxypeptidase (PRCP))
- Alternative Name
- PRCP (PRCP Products)
- Synonyms
- OI13 ELISA Kit, PCOLC ELISA Kit, PCP ELISA Kit, PCP2 ELISA Kit, TLD ELISA Kit, humpcp ELISA Kit, MGC145178 ELISA Kit, 2510048K03Rik ELISA Kit, 2610104A14Rik ELISA Kit, AI451719 ELISA Kit, HUMPCP ELISA Kit, zgc:91816 ELISA Kit, bone morphogenetic protein 1 ELISA Kit, prolylcarboxypeptidase ELISA Kit, lysosomal Pro-X carboxypeptidase ELISA Kit, prolylcarboxypeptidase (angiotensinase C) ELISA Kit, BMP1 ELISA Kit, prcp ELISA Kit, LOC5579822 ELISA Kit, CpipJ_CPIJ006123 ELISA Kit, CpipJ_CPIJ006124 ELISA Kit, Prcp ELISA Kit, PRCP ELISA Kit
- Gene ID
- 5547
- UniProt
- P42785
-