×
For best experience we recommend to activate Javascript in your browser.
SEMA6A ELISA Kit
SEMA6A
Reactivity: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Product Details SEMA6A ELISA Kit
(hide)
Target
See all SEMA6A ELISA Kits
SEMA6A
(Sema Domain, Transmembrane Domain (TM), and Cytoplasmic Domain, (Semaphorin) 6A (SEMA6A))
Reactivity
All reactivities for SEMA6A elisa kits
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Application
ELISA
Purpose
Custom Human Semaphorin 6A ELISA Kit.
Sample Type
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificity
This ELISA antibody pair recognizes human Semaphorin 6A. Other species not yet determined.
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Alternatives
(show)
Application Details
(hide)
Sample Volume
100 μL
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
(hide)
Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for SEMA6A
(hide)
Target
See all SEMA6A ELISA Kits
SEMA6A
(Sema Domain, Transmembrane Domain (TM), and Cytoplasmic Domain, (Semaphorin) 6A (SEMA6A))
Alternative Name
Semaphorin 6A (SEMA6A Products )
Synonyms
fj46c12 ELISA Kit, wu:fj46c12 ELISA Kit, zgc:66106 ELISA Kit, SEMA6A ELISA Kit, HT018 ELISA Kit, SEMA ELISA Kit, SEMA6A1 ELISA Kit, SEMAQ ELISA Kit, VIA ELISA Kit, 9330158E07 ELISA Kit, A730020P05Rik ELISA Kit, AI851735 ELISA Kit, Sema6A-1 ELISA Kit, Semaq ELISA Kit, VIa ELISA Kit, b2b997Clo ELISA Kit, sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A ELISA Kit, semaphorin 6A ELISA Kit, sema6a ELISA Kit, SEMA6A ELISA Kit, Sema6a ELISA Kit
Gene ID
57556
UniProt
Q9H2E6
Recently viewed
(hide)