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SERPINB6 ELISA Kit
SERPINB6
Reactivity: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Product Details SERPINB6 ELISA Kit
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Target
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SERPINB6
(serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 6 (SERPINB6))
Reactivity
All reactivities for SERPINB6 elisa kits
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Application
ELISA
Purpose
Custom Human Serpin B6 ELISA Kit.
Sample Type
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Application Details
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Sample Volume
100 μL
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for SERPINB6
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Target
See all SERPINB6 ELISA Kits
SERPINB6
(serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 6 (SERPINB6))
Alternative Name
Serpin B6 (SERPINB6 Products )
Synonyms
Serpinb6a ELISA Kit, cap ELISA Kit, dfnb91 ELISA Kit, mstp057 ELISA Kit, pi6 ELISA Kit, pti ELISA Kit, serpinb8 ELISA Kit, spi3 ELISA Kit, 4930482L21Rik ELISA Kit, D330015H01Rik ELISA Kit, PI-6 ELISA Kit, Serpinb6 ELISA Kit, Spi3 ELISA Kit, ovalbumin ELISA Kit, CAP ELISA Kit, DFNB91 ELISA Kit, MSTP057 ELISA Kit, PI6 ELISA Kit, PTI ELISA Kit, SPI3 ELISA Kit, serpin family B member 6A ELISA Kit, serpin family B member 6 ELISA Kit, serine (or cysteine) peptidase inhibitor, clade B, member 6a ELISA Kit, serpin peptidase inhibitor, clade B (ovalbumin), member 6 ELISA Kit, Serpinb6a ELISA Kit, serpinb6 ELISA Kit, SERPINB6 ELISA Kit
Gene ID
5269
UniProt
P35237
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