TRAIL ELISA Kit

Catalog No. ABIN4884871

Product Details TRAIL ELISA Kit

Target: TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 40-10.000 pg/mL

Minimum Detection Limit: 40 pg/mL

Application: ELISA

Purpose: Human TRAIL (TNFSF10) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Sample Type: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificity: This ELISA antibody pair detects human TRAIL. Other species not determined.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples2 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

References for TRAIL ELISA Kit (ABIN4884871)

Yi, Elson, Mitsuhashi, Jacobs, Hollovary, Budd, Spiro, Triozzi, Borden: "Phosphatase inhibitor, sodium stibogluconate, in combination with interferon (IFN) alpha 2b: phase I trials to identify pharmacodynamic and clinical effects." in: Oncotarget, Vol. 2, Issue 12, pp. 1155-64, (2012) (PubMed).

Target Details for TRAIL

Target: TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))

Alternative Name: TRAIL (TNFSF10 Products)

Synonyms: APO2L ELISA Kit, Apo-2L ELISA Kit, CD253 ELISA Kit, TL2 ELISA Kit, TRAIL ELISA Kit, A330042I21Rik ELISA Kit, AI448571 ELISA Kit, APO-2L ELISA Kit, Ly81 ELISA Kit, Trail ELISA Kit, tl2 ELISA Kit, apo2l ELISA Kit, cd253 ELISA Kit, trail ELISA Kit, apo-2l ELISA Kit, trail1 ELISA Kit, xtrail1 ELISA Kit, tnfsf10l2 ELISA Kit, zgc:92320 ELISA Kit, TNLG6A ELISA Kit, TNF superfamily member 10 ELISA Kit, tumor necrosis factor (ligand) superfamily, member 10 ELISA Kit, tumor necrosis factor superfamily member 10 ELISA Kit, TNF superfamily member 10 S homeolog ELISA Kit, TNFSF10 ELISA Kit, Tnfsf10 ELISA Kit, tnfsf10.S ELISA Kit, tnfsf10 ELISA Kit

Gene ID: 8743

UniProt: P50591

Pathways: Apoptosis, Positive Regulation of Endopeptidase Activity