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PGLYRP1 ELISA Kit
PGLYRP1
Reactivity: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Plasma, Serum
Product Details PGLYRP1 ELISA Kit
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Target
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PGLYRP1
(Peptidoglycan Recognition Protein 1 (PGLYRP1))
Reactivity
All reactivities for PGLYRP1 elisa kits
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Application
ELISA
Purpose
Custom Human PGRPs SpeedELISA Kit.
Brand
SpeedELISA
Sample Type
Plasma, Cell Culture Supernatant, Serum
Analytical Method
Quantitative
Characteristics
Compressed workflow with only one wash step and three incubation steps 3 hours total processing time Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Streptavidin-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Capture Antibody HRP Conjugated Detection Antibody TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Application Details
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Sample Volume
100 μL
Assay Time
3 h
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 70 μL of the Antibody Cocktail to each well. Incubate 10 minutes at RT. Add 50 μL of sample or standard to each well. Incubate 2 h at RT. Wash Wells Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage
-20 °C
Storage Comment
May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
Expiry Date
6 months
Target Details for PGLYRP1
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Target
See all PGLYRP1 ELISA Kits
PGLYRP1
(Peptidoglycan Recognition Protein 1 (PGLYRP1))
Alternative Name
PGRPs (PGLYRP1 Products )
Synonyms
PGLYRP1 ELISA Kit, PGLYRP ELISA Kit, pgrp ELISA Kit, tag7 ELISA Kit, pgrps ELISA Kit, pglyrp ELISA Kit, pgrp-s ELISA Kit, tnfsf3l ELISA Kit, MGC108330 ELISA Kit, MGC108469 ELISA Kit, PGRP-SC1a ELISA Kit, si:dkey-113g17.4 ELISA Kit, si:ch211-202c21.3 ELISA Kit, PGRP ELISA Kit, PGRP-S ELISA Kit, PGRPS ELISA Kit, TAG7 ELISA Kit, TNFSF3L ELISA Kit, Pglyrp ELISA Kit, Tag7 ELISA Kit, Tasg7 ELISA Kit, Tnfsf3l ELISA Kit, Pgrp ELISA Kit, peptidoglycan recognition protein 1 ELISA Kit, peptidoglycan recognition protein 1 L homeolog ELISA Kit, peptidoglycan recognition protein 5 ELISA Kit, PGLYRP1 ELISA Kit, pglyrp1.L ELISA Kit, pglyrp1 ELISA Kit, pglyrp5 ELISA Kit, Pglyrp1 ELISA Kit
Gene ID
8993
UniProt
O75594
Pathways
Activation of Innate immune Response
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