Vitronectin ELISA Kit

Catalog No. ABIN4885378

Product Details Vitronectin ELISA Kit

Target: Vitronectin (VTN)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Custom Human Vitronectin SpeedELISA Kit.

Brand: SpeedELISA

Sample Type: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Characteristics:

• Compressed workflow with only one wash step and three incubation steps
• 3 hours total processing time
• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Streptavidin-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Capture Antibody
• HRP Conjugated Detection Antibody
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Sample Volume: 100 μL

Assay Time: 3 h

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 70 μL of the Antibody Cocktail to each well.
3. Incubate 10 minutes at RT.
4. Add 50 μL of sample or standard to each well.
5. Incubate 2 h at RT.
6. Wash Wells
7. Add 100 μL of TMB One-Step Substrate Reagent to each well.
8. Incubate 30 min at RT.
9. Add 50 μL of Stop Solution to each well.
10. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.
Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.

Expiry Date: 6 months

Target Details for Vitronectin

Target: Vitronectin (VTN)

Alternative Name: Vitronectin (VTN Products)

Synonyms: VTN ELISA Kit, DKFZp470F0511 ELISA Kit, LOC100228142 ELISA Kit, vtn ELISA Kit, zgc:112277 ELISA Kit, V75 ELISA Kit, VN ELISA Kit, VNT ELISA Kit, AI256434 ELISA Kit, Vn ELISA Kit, Aa1018 ELISA Kit, vitronectin ELISA Kit, vitronectin a ELISA Kit, vitronectin b ELISA Kit, vitronectin L homeolog ELISA Kit, VTN ELISA Kit, vtn ELISA Kit, vtna ELISA Kit, vtnb ELISA Kit, CpipJ_CPIJ016691 ELISA Kit, vtn.L ELISA Kit, Vtn ELISA Kit

Gene ID: 7448

UniProt: P04004

Pathways: Autophagy, Smooth Muscle Cell Migration