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TNFRSF21 ELISA Kit
TNFRSF21
Reactivity: Mouse
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Product Details TNFRSF21 ELISA Kit
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Target
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TNFRSF21
(Tumor Necrosis Factor Receptor Superfamily, Member 21 (TNFRSF21))
Reactivity
All reactivities for TNFRSF21 elisa kits
Mouse
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Application
ELISA
Purpose
Custom Mouse Death Receptor 6 ELISA Kit.
Sample Type
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificity
The antibody pair provided in this kit recognizes Mouse Death Receptor 6.
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Application Details
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Sample Volume
100 μL
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for TNFRSF21
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Target
See all TNFRSF21 ELISA Kits
TNFRSF21
(Tumor Necrosis Factor Receptor Superfamily, Member 21 (TNFRSF21))
Alternative Name
DR6 (TNFRSF21 Products )
Synonyms
TNFRSF21 ELISA Kit, tnfrsf21 ELISA Kit, MGC146356 ELISA Kit, BM-018 ELISA Kit, CD358 ELISA Kit, DR6 ELISA Kit, AA959878 ELISA Kit, R74815 ELISA Kit, TR7 ELISA Kit, dr6 ELISA Kit, im:6795346 ELISA Kit, wu:fa55e01 ELISA Kit, wu:fb02e11 ELISA Kit, wu:fc29a09 ELISA Kit, wu:fi27h08 ELISA Kit, TNF receptor superfamily member 21 ELISA Kit, tumor necrosis factor receptor superfamily member 21 ELISA Kit, tumor necrosis factor receptor superfamily, member 21 ELISA Kit, TNFRSF21 ELISA Kit, tnfrsf21 ELISA Kit, Tnfrsf21 ELISA Kit
Gene ID
94185
UniProt
Q9EPU5
Pathways
Regulation of Lipid Metabolism by PPARalpha
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