This immunoassay kit allows for the in vitro quantitative determination of Human HIF1Alpha concentrations in serum, plasma and other biological fluids.
HIF1A
Reactivity: Human
Colorimetric
DNA-Binding ELISA
Cell Lysate, Nuclear Extract
Sample Volume
100 μL
Assay Time
3 - 4 h
Plate
Pre-coated
Protocol
This assay employs an antibody specific for Anti-human HIF1Alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and HIF1Alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human HIF1Alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of HIF1Alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.