IL12 ELISA Kit
Quick Overview for IL12 ELISA Kit (ABIN5026962)
Target
See all IL12 ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
-
-
Minimum Detection Limit
- 15.6 pg/mL
-
Purpose
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology.
-
Analytical Method
- Quantitative
-
Sensitivity
- 9.4 pg/mL
-
Characteristics
- This immunoassay kit allows for the in vitro quantitative determination of Human IL-12 (p70) concentrations in serum, plasma. Detection Range: 15.6 - 1000 pg/mL Sensitivity: < 9.4 pg/mL
-
Components
- plate, standard, diluent, antibodies
-
Material not included
- pipettes, tubes, reader, incubator
-
-
Interleukin 12 (IL12) ELISA Kit
IL12 Reactivity: Chicken Colorimetric Competition ELISA 0.78 pg/mL - 200 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 12 (IL12) ELISA KitIL12 Reactivity: Rabbit Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 12 (IL12) ELISA KitIL12 Reactivity: Rat Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 12 (IL12) ELISA KitIL12 Reactivity: Pig Colorimetric Sandwich ELISA 0.45 ng/mL - 30 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Interleukin 12 (IL12) ELISA KitIL12 Reactivity: Dog Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Interleukin 12 (IL12) ELISA KitIL12 Reactivity: Guinea Pig Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 12 (IL12) ELISA KitIL12 Reactivity: Human Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 12 (IL12) ELISA KitIL12 Reactivity: Monkey Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 12 (IL12) ELISA KitIL12 Reactivity: Rat Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 12 (IL12) ELISA KitIL12 Reactivity: Sheep Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
-
-
Sample Volume
- 100 μL
-
Assay Time
- 3 - 4 h
-
Plate
- Pre-coated
-
Protocol
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human IL-12 (p70) antibody was pre-coated into 96-well plates. Biotin conjugated anti-human IL-12 (p70) detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IL-12 (p70) amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450 nm in a microplate reader. Concentration of Human IL-12 (p70) can be calculated using the standard curve.
-
Restrictions
- For Research Use only
-
-
-
Precaution of Use
- protect your eyes
-
Storage
- 4 °C
-
Storage Comment
- Please refer to the Manual
-
-
- IL12 (Interleukin 12 (IL12))
-
Alternative Name
- IL-12 (p70) (Interleukin 12, p70)
-
Pathways
- JAK-STAT Signaling, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome
Target See all IL12 ELISA Kits
-
