C3 ELISA Kit

Catalog No. ABIN5067885

Product Details C3 ELISA Kit

Target: C3 (Complement Component 3 (C3))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Complement C3 ELISA Kit (Mouse) is for the quantitative detection of Complement C3 in mouse serum, plasma, tissue homogenates and other biological fluids.

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 1.875 ng/mL

Characteristics: C3 (Complement C3) ELISA Kit (Mouse)

Components:

• *354375A: Microtiter Strips, 1x96 wells
• *354375B: Standard, 2x1vial
• 354375C: Sample/Standard Dilution Buffer, 1x20ml
• 354375D: Antibody (Biotin) (Concentrated), 1x120ul
• 354375E: Antibody Dilution Buffer, 1x10ml
• 354375F: Streptavidin-HRP Conjugate (SABC), 1x120ul
• 354375G: SABC Dilution Buffer, 1x10ml
• 354375H: TMB Substrate, 1x10ml
• 354375J: Stop Solution, 1x10ml
• 354375K: Wash Buffer (25X), 1x30ml

Application Details

Plate: Pre-coated

Protocol: Principle:

• The microtiter plate provided in this kit has been pre-coated with an antibody specific to C3. Standards and samples are added to the appropriate microtiter plate wells followed by a biotin-conjugated antibody specific to C3. Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C3, Biotin-conjugated antibody and enzyme-conjugated Streptavidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of C3 in the sample is then determined by comparing the O.D. of the sample to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: 4°C/-20°C

Target Details for C3

Target: C3 (Complement Component 3 (C3))

Alternative Name: C3 (Complement C3) (C3 Products)

Synonyms: AHUS5 ELISA Kit, ARMD9 ELISA Kit, ASP ELISA Kit, C3a ELISA Kit, C3b ELISA Kit, CPAMD1 ELISA Kit, AI255234 ELISA Kit, HSE-MSF ELISA Kit, Plp ELISA Kit, C3 ELISA Kit, C3-1 ELISA Kit, sb:cb26 ELISA Kit, si:dkey-76b14.4 ELISA Kit, wu:fi34b03 ELISA Kit, wu:fj86e11 ELISA Kit, C3d ELISA Kit, asp ELISA Kit, cpamd1 ELISA Kit, c3 ELISA Kit, complement C3 ELISA Kit, complement component 3 ELISA Kit, complement component c3a, duplicate 1 ELISA Kit, complement component 3 L homeolog ELISA Kit, complement component C3 ELISA Kit, complement C3-like ELISA Kit, C3 ELISA Kit, c3a.1 ELISA Kit, c3.L ELISA Kit, LOC443475 ELISA Kit, c3 ELISA Kit, c3-1 ELISA Kit, LOC100060539 ELISA Kit

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling