CD74 ELISA Kit

Catalog No. ABIN5067920

Product Details CD74 ELISA Kit

Target: CD74 (HLA-DR-gamma (CD74))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: The CD74 (HLA Class II Histocompatibility Antigen gamma Chain) ELISA Kit (Human) is a quantitative sandwich assay for the detection of CD74 in human serum, plasma, tissue homogenates and other biological fluids.

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: > 0.375 ng/mL

Characteristics: CD74 (HLA Class II Histocompatibility Antigen gamma Chain) ELISA Kit (Human)

Components:

• *354655A: Microtiter Strips, 8x12 wells.
• *354655B: Standard, 2 vials
• 354655C: Sample/Standard Dilution Buffer, 1x20ml
• 354655D: Antibody-Biotin (Concentrated), 1x120ul
• 354655E: Antibody Dilution Buffer, 1x10ml
• 354655F: Streptavidin-HRP Conjugate (SABC), 1x120ul
• 354655G: SABC Dilution Buffer, 1x10ml
• 354655H: TMB Substrate, 1x10ml
• 354655J: Stop Solution, 1x10ml
• 354655K: Wash Buffer (25X), 1x30ml

Application Details

Plate: Pre-coated

Protocol: Principle:

• The microtiter plate provided in this kit has been pre-coated with an antibody specific to CD74. Standards and samples are added to the appropriate microtiter plate wells followed by a biotin-conjugated antibody specific to CD74. Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CD74, Biotin-conjugated antibody and enzyme-conjugated Streptavidin complex will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CD74 in the sample is then determined by comparing the O.D. of the sample to the standard curve.

Assay Precision: Precision:

• Intra-Assay CV: <8%
• Inter-Assay CV: <10%

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: 4°C/-20°C

Target Details for CD74

Target: CD74 (HLA-DR-gamma (CD74))

Alternative Name: CD74 (HLA Class II Histocompatibility Antigen gamma Chain) (CD74 Products)

Synonyms: DHLAG ELISA Kit, HLADG ELISA Kit, II ELISA Kit, Ia-GAMMA ELISA Kit, CLIP ELISA Kit, Ii ELISA Kit, INVG34 ELISA Kit, Iclp-1 ELISA Kit, iclp1 ELISA Kit, wu:fb11f09 ELISA Kit, wu:fb12a01 ELISA Kit, wu:fb13d06 ELISA Kit, wu:fk94e07 ELISA Kit, Iclp-2 ELISA Kit, cd74 ELISA Kit, iclp2 ELISA Kit, zgc:123161 ELISA Kit, CD74 molecule ELISA Kit, CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) ELISA Kit, CD74 molecule, major histocompatibility complex, class II invariant chain a ELISA Kit, CD74 molecule, major histocompatibility complex, class II invariant chain b ELISA Kit, CD74 ELISA Kit, Cd74 ELISA Kit, cd74a ELISA Kit, cd74b ELISA Kit

Pathways: Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Negative Regulation of intrinsic apoptotic Signaling, Cancer Immune Checkpoints