IFI16 ELISA Kit

Catalog No. ABIN5067979

Product Details IFI16 ELISA Kit

Target: IFI16 (Interferon, gamma-Inducible Protein 16 (IFI16))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: The IFI16 (Gamma-interferon-inducible protein 16) ELISA Kit (Human) is a quantitative sandwich assay for the detection of IFI16 in human serum, plasma, tissue homogenates and other biological fluids.

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: > 0.094 ng/mL

Characteristics: IFI16 (Gamma-interferon-inducible protein 16) ELISA Kit (Human)

Components:

• *356457A: Microtiter Strips, 8x12 wells.
• *356457B: Standard, 2 vials
• 356457C: Sample/Standard Dilution Buffer, 1x20ml
• 356457D: Antibody-Biotin (Concentrated), 1x120ul
• 356457E: Antibody Dilution Buffer, 1x10ml
• 356457F: Streptavidin-HRP Conjugate (SABC), 1x120ul
• 356457G: SABC Dilution Buffer, 1x10ml
• 356457H: TMB Substrate, 1x10ml
• 356457J: Stop Solution, 1x10ml
• 356457K: Wash Buffer (25X), 1x30ml

Application Details

Plate: Pre-coated

Protocol: Principle:

• The microtiter plate provided in this kit has been pre-coated with an antibody specific to IFI16 . Standards and samples are added to the appropriate microtiter plate wells followed by a biotin-conjugated antibody specific to IFI16. Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IFI16, Biotin-conjugated antibody and enzyme-conjugated Streptavidin complex will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of IFI16 in the sample is then determined by comparing the O.D. of the sample to the standard curve.

Assay Precision: Precision:

• Intra-Assay CV: <8%
• Inter-Assay CV: <10%

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: 4°C/-20°C

Target Details for IFI16

Target: IFI16 (Interferon, gamma-Inducible Protein 16 (IFI16))

Alternative Name: IFI16 (Gamma-interferon-inducible protein 16) (IFI16 Products)

Synonyms: IFNGIP1 ELISA Kit, PYHIN2 ELISA Kit, IFI16 ELISA Kit, Ifitm3l ELISA Kit, Ifitml ELISA Kit, interferon gamma inducible protein 16 ELISA Kit, interferon induced transmembrane protein 2 ELISA Kit, myeloid cell nuclear differentiation antigen-like protein ELISA Kit, interferon-activable protein 203 ELISA Kit, IFI16 ELISA Kit, Ifitm2 ELISA Kit, LOC100156073 ELISA Kit, LOC488622 ELISA Kit, Ifi16 ELISA Kit

Pathways: Activation of Innate immune Response, Positive Regulation of Endopeptidase Activity, Autophagy, Inflammasome