PDGF-BB Homodimer ELISA Kit

Catalog No. ABIN5067997

Product Details

Target: PDGF-BB Homodimer

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: The Mouse Platelet-Derived Growth Factor-BB (PDGF-BB) ELISA Kit is a quantitative sandwich assay for the detection of PDGF-BB in mouse serum, plasma, tissue homogenates and other biological fluids.

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 23.438 pg/mL

Characteristics: PDGF-BB (Platelet-Derived Growth Factor-BB) ELISA Kit (Mouse)

Components:

• *357966A: Microtiter Strips, 8x12 wells.
• *357966B: Standard, 2 vials
• 357966C: Sample/Standard Dilution Buffer, 1x20ml
• 357966D: Antibody-Biotin (Concentrated), 1x120ul
• 357966E: Antibody Dilution Buffer, 1x10ml
• 357966F: Streptavidin-HRP Conjugate (SABC), 1x120ul
• 357966G: SABC Dilution Buffer, 1x10ml
• 357966H: TMB Substrate, 1x10ml
• 357966J: Stop Solution, 1x10ml
• 357966K: Wash Buffer (25X), 1x30ml

Application Details

Plate: Pre-coated

Protocol: Principle:

• The microtiter plate provided in this kit has been pre-coated with an antibody specific to PDGF-BB. Standards and samples are added to the appropriate microtiter plate wells followed by a biotin-conjugated antibody specific to PDGF-BB. Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PDGF-BB, Biotin-conjugated antibody and enzyme-conjugated Streptavidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PDGF-BB in the sample is then determined by comparing the O.D. of the sample to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: 4°C/-20°C

Target Details

Target: PDGF-BB Homodimer

Alternative Name: PDGF-BB (Platelet-Derived Growth Factor-BB)

Synonyms: PDGF-2 ELISA Kit, PDGF2 ELISA Kit, SIS ELISA Kit, SSV ELISA Kit, c-sis ELISA Kit, PDGF-B ELISA Kit, Sis ELISA Kit, platelet derived growth factor subunit B ELISA Kit, platelet derived growth factor, B polypeptide ELISA Kit, PDGFB ELISA Kit, Pdgfb ELISA Kit