NEDD9 ELISA Kit

Catalog No. ABIN5068005

Product Details NEDD9 ELISA Kit

Target: NEDD9 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: The NEDD9 (Enhancer of filamentation 1) ELISA Kit (Human) is a quantitative sandwich assay for the detection of NEDD9 in human serum, plasma, tissue homogenates and other biological fluids.

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: > 0.094 ng/mL

Characteristics: NEDD9 (Enhancer of filamentation 1) ELISA Kit (Human)

Components:

• *357566A: Microtiter Strips, 8x12 wells.
• *357566B: Standard, 2 vials
• 357566C: Sample/Standard Dilution Buffer, 1x20ml
• 357566D: Antibody-Biotin (Concentrated), 1x120ul
• 357566E: Antibody Dilution Buffer, 1x10ml
• 357566F: Streptavidin-HRP Conjugate (SABC), 1x120ul
• 357566G: SABC Dilution Buffer, 1x10ml
• 357566H: TMB Substrate, 1x10ml
• 357566J: Stop Solution, 1x10ml
• 357566K: Wash Buffer (25X), 1x30ml

Application Details

Plate: Pre-coated

Protocol: Principle:

• The microtiter plate provided in this kit has been pre-coated with an antibody specific to NEDD9. Standards and samples are added to the appropriate microtiter plate wells followed by a biotin-conjugated antibody specific to NEDD9. Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NEDD9, Biotin-conjugated antibody and enzyme-conjugated Streptavidin complex will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NEDD9 in the sample is then determined by comparing the O.D. of the sample to the standard curve.

Assay Precision: Precision:

• Intra-Assay CV: <8%
• Inter-Assay CV: <10%

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: 4°C/-20°C

Target Details for NEDD9

Target: NEDD9 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9))

Alternative Name: NEDD9 (Enhancer of filamentation 1) (NEDD9 Products)

Synonyms: NEDD9 ELISA Kit, CAS-L ELISA Kit, CAS2 ELISA Kit, CASL ELISA Kit, CASS2 ELISA Kit, HEF1 ELISA Kit, Cas-L ELISA Kit, CasL ELISA Kit, E230025G09Rik ELISA Kit, neural precursor cell expressed, developmentally down-regulated 9 ELISA Kit, neural precursor cell expressed, developmentally down-regulated gene 9 ELISA Kit, NEDD9 ELISA Kit, Nedd9 ELISA Kit

UniProt: Q14511