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eIF4EBP1 ELISA Kit

EIF4EBP1 Reactivity: Mouse, Human pThr36 Colorimetric Cell ELISA Adherent Cell Culture
Catalog No. ABIN5526687
  • Target See all eIF4EBP1 (EIF4EBP1) ELISA Kits
    eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))
    Binding Specificity
    pThr36, total
    Reactivity
    • 7
    • 6
    • 5
    • 1
    Mouse, Human
    Detection Method
    Colorimetric
    Method Type
    Cell ELISA
    Application
    ELISA
    Purpose
    Human and Mouse Phospho-4E-BP1 (Thr36) and Total 4E-BP1 Cell-Based ELISA Cell-Based ELISA Kit. This assay semi-quantitatively measures 4E-BP1 phosphorylated at Threonine-36 as well as total 4E-BP1 in adherent cell lines.
    Sample Type
    Adherent Cell Culture
    Analytical Method
    Semi-Quantitative
    Specificity
    This ELISA kit recognizes Human and Mouse 4E-BP1 phosphorylated at site Threonine-36 as well as total 4E-BP1.
    Characteristics
    • Rapidly measure phosphorylated protein in adherent cell lines
    • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
    • No sample lysis is needed
    • Compatible with a standard ELISA plate reader
    Components
    • Uncoated 96-well Strip Microplate
    • Wash Buffers
    • Fixing Solution
    • Quenching Buffer
    • Blocking Buffer
    • Anti-phospho antibody
    • Anti-pan antibody
    • HRP-Conjugated Secondary Antibody
    • TMB One-Step Substrate
    • Stop Solution
    Material not included
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
    Top Product
    Discover our top product EIF4EBP1 ELISA Kit
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Expiry Date
    6 months
  • Target See all eIF4EBP1 (EIF4EBP1) ELISA Kits
    eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))
    Alternative Name
    4E-BP1 (EIF4EBP1 Products)
    Synonyms
    4E-BP1 ELISA Kit, 4EBP1 ELISA Kit, BP-1 ELISA Kit, PHAS-I ELISA Kit, 4e-bp1 ELISA Kit, AA959816 ELISA Kit, 153432_at ELISA Kit, 43-BP ELISA Kit, 4E-BP ELISA Kit, 4EBP ELISA Kit, 4e-bp ELISA Kit, 4eBP ELISA Kit, 4ebp ELISA Kit, BcDNA.HL08053 ELISA Kit, BcDNA:HL08053 ELISA Kit, CG8846 ELISA Kit, CT8705 ELISA Kit, Dm 4E-BP ELISA Kit, Dmel\\CG8846 ELISA Kit, Phas1 ELISA Kit, Thor/EIF4EBP ELISA Kit, anon-WO0172774.158 ELISA Kit, d4E-BP ELISA Kit, d4E-BP1 ELISA Kit, d4EBP ELISA Kit, d4e-bp ELISA Kit, d4eBP ELISA Kit, dthor ELISA Kit, eIF4E-BP ELISA Kit, l(2)06270 ELISA Kit, l(2)k07736 ELISA Kit, l(2)k13517 ELISA Kit, pp20 ELISA Kit, thor ELISA Kit, EIF4EBP1 ELISA Kit, fc04h09 ELISA Kit, wu:fc04h09 ELISA Kit, zgc:64137 ELISA Kit, eukaryotic translation initiation factor 4E binding protein 1 ELISA Kit, CG8846 gene product from transcript CG8846-RA ELISA Kit, EIF4EBP1 ELISA Kit, Eif4ebp1 ELISA Kit, Thor ELISA Kit, eif4ebp1 ELISA Kit
    Gene ID
    1978
    UniProt
    Q13541, Q60876
    Pathways
    MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Regulation of Cell Size, BCR Signaling
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