ATM ELISA Kit

Catalog No. ABIN5526689

Product Details ATM ELISA Kit

Target: ATM (Ataxia Telangiectasia Mutated (ATM))

Binding Specificity: pSer1981

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Purpose: Human Phospho-ErbB3 (Tyr1262) and Total ErbB3 Cell-Based ELISA Cell-Based ELISA Kit. This assay semi-quantitatively measures ATM phosphorylated at Serine-1981 as well as total ATM in adherent cell lines.

Sample Type: Adherent Cell Culture

Analytical Method: Semi-Quantitative

Specificity: This ELISA kit recognizes Human ATM phosphorylated at site Serine-1981 as well as total ATM.

Characteristics:

• Rapidly measure phosphorylated protein in adherent cell lines
• Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
• No sample lysis is needed
• Compatible with a standard ELISA plate reader

Components:

• Uncoated 96-well Strip Microplate
• Wash Buffers
• Fixing Solution
• Quenching Buffer
• Blocking Buffer
• Anti-phospho antibody
• Anti-pan antibody
• HRP-Conjugated Secondary Antibody
• TMB One-Step Substrate
• Stop Solution

Material not included:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Expiry Date: 6 months

Target Details for ATM

Target: ATM (Ataxia Telangiectasia Mutated (ATM))

Alternative Name: ATM (ATM Products)

Synonyms: ATM ELISA Kit, Atm ELISA Kit, CG6535 ELISA Kit, Dmel\\CG6535 ELISA Kit, Tefu ELISA Kit, atm ELISA Kit, atm/tefu ELISA Kit, dATM ELISA Kit, tef ELISA Kit, Xatm ELISA Kit, at1 ELISA Kit, atdc ELISA Kit, tel1 ELISA Kit, telo1 ELISA Kit, AT1 ELISA Kit, ATA ELISA Kit, ATC ELISA Kit, ATD ELISA Kit, ATDC ELISA Kit, ATE ELISA Kit, TEL1 ELISA Kit, TELO1 ELISA Kit, AI256621 ELISA Kit, C030026E19Rik ELISA Kit, telomere fusion ELISA Kit, ATM serine/threonine kinase L homeolog ELISA Kit, ATM serine/threonine kinase ELISA Kit, ataxia telangiectasia mutated ELISA Kit, ataxia telangiectasia mutated (atm) ELISA Kit, serine/threonine-protein kinase ATM ELISA Kit, tefu ELISA Kit, atm.L ELISA Kit, atm ELISA Kit, ATM ELISA Kit, EDI_100660 ELISA Kit, CpipJ_CPIJ001772 ELISA Kit, BDBG_08252 ELISA Kit, PAAG_02532 ELISA Kit, MCYG_05088 ELISA Kit, VDBG_06833 ELISA Kit, ACLA_015700 ELISA Kit, LOC5565620 ELISA Kit, MGYG_07634 ELISA Kit, PGTG_14279 ELISA Kit, Atm ELISA Kit

Gene ID: 472

UniProt: Q13315

Pathways: p53 Signaling, Apoptosis, DNA Damage Repair, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus