Salmonella Abortus Ovis IgG ELISA Kit
Reactivity: Sheep
Colorimetric
Competition ELISA
Plasma, Serum
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- Target
- Salmonella Abortus Ovis IgG
- Reactivity
- Sheep
- Detection Method
- Colorimetric
- Method Type
- Competition ELISA
- Application
- ELISA
- Purpose
- Enzyme-linked immunosorbent assay (ELISA) for the detection of anti-Salmonella abortus ovis IgG in sheep serum and plasma for the diagnosis of abortive salmonellosis infection and evaluation of antibody response to vaccination.
- Sample Type
- Plasma, Serum
- Analytical Method
- Qualitative
- Components
- - Microtiter strips (12 x 8 well strips): 1 microplate coated with S. abortus ovis LPS, with preservative - Buffer A: 50ml ready to use, with preservative - Buffer B (Wash Buffer 10X concentrate): 100ml, to dilute to 1X with distilled water, with preservative - HRP-conjugated secondary antibody: to dilute in Buffer A as indicated on the label - ABTS Solution: 13ml, ready to use with preservative
- Material not included
- Microplate reader capable of measuring absorbance at 415 nm Thermomixer, shaking water bath or rocking platform at 37 °C Precision pipettes and pipette tips Glass or plastic pipettes Deionized or distilled water Multi-channel pipette, semi-automated or automated microplate washer 1000 mL graduated cylinder for preparation of 1X Wash Buffer Vortex mixer Glass tubes
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- Plate
- Pre-coated
- Restrictions
- For Research Use only
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- Precaution of Use
- The buffer C1 provided with this kit is H2O2 solution 30% m/m (110 volumes) that causes burns
- Storage
- 4 °C
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- Target
- Salmonella Abortus Ovis IgG
- Target Type
- Antibody
- Background
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Salmonella enteritidis subsp. enteritidis ser. abortus ovis, a sheep-adapted serotype, causes an infectious disease with abortion as the main symptom sometimes accompanied with mortality of lambs at term.
Salmonella abortus ovis infections can be found worldwide, but are particularly common in Europe and Western Asia.
Diagnosis is made by culture of placenta, fetus, or uterine discharge. Isolation of aborting ewes and destruction of contaminated bedding and of all products of abortion reduce contamination. Prevention is mainly based on vaccination and annual vaccination with dead or living vaccines is advisable in endemic areas.
Serological research of antibodies against S. abortus ovis could have a fundamental importance when the infected sheep showing no excretion of the pathogen. Moreover a simple, quick and reliable ELISA test could made easier the infection diagnosis and the herds screening. Obviously the assay can be also used to check the immunity response to S. abortus ovis vaccination.
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