Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

ARG2 ELISA Kit

ARG2 Reactivity: Mouse Colorimetric Sandwich ELISA 78.12 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Catalog No. ABIN5652002
  • Target See all ARG2 ELISA Kits
    ARG2 (Arginase, Type II (ARG2))
    Reactivity
    • 3
    • 1
    • 1
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    78.12 pg/mL - 5000 pg/mL
    Minimum Detection Limit
    78.12 pg/mL
    Application
    ELISA
    Sample Type
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Arginase II (Arg2). No significant cross-reactivity or interference between Arginase II (Arg2) and analogues was observed.
    Sensitivity
    34 pg/mL
    Featured
    Discover our best selling ARG2 ELISA Kit
    Top Product
    Discover our top product ARG2 ELISA Kit
  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    3 h
    Plate
    Pre-coated
    Protocol
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arginase II (Arg2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Arginase II (Arg2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arginase II (Arg2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Arginase II (Arg2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Arginase II (Arg2) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Arginase II (Arg2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target See all ARG2 ELISA Kits
    ARG2 (Arginase, Type II (ARG2))
    Alternative Name
    Arginase II (ARG2 Products)
    Synonyms
    AII ELISA Kit, AU022422 ELISA Kit, zgc:65793 ELISA Kit, zgc:85630 ELISA Kit, wu:fb67g02 ELISA Kit, ARG2 ELISA Kit, LeARG2 ELISA Kit, arg1 ELISA Kit, arg3 ELISA Kit, arg2 ELISA Kit, arg2-c ELISA Kit, arg2-b ELISA Kit, arginase 2 ELISA Kit, arginase type II ELISA Kit, arginase 2 L homeolog ELISA Kit, arginase 2 S homeolog ELISA Kit, ARG2 ELISA Kit, Arg2 ELISA Kit, arg2 ELISA Kit, arg2.L ELISA Kit, arg2.S ELISA Kit
    Background

    Gene Name: Arginase II

    Gene Aliases: Kidney-type arginase, Non-hepatic arginase

    Gene ID
    11847
    UniProt
    O08691
You are here:
Support