Aromatase ELISA Kit

Catalog No. ABIN5652018

Product Details Aromatase ELISA Kit

Target: Aromatase (CYP19A1) (Cytochrome P450, Family 19, Subfamily A, Polypeptide 1 (CYP19A1))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56 ng/mL - 100 ng/mL

Minimum Detection Limit: 1.56 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Aromatase (ARO). No significant cross-reactivity or interference between Aromatase (ARO) and analogues was observed.

Sensitivity: 0.62 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aromatase (ARO). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aromatase (ARO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aromatase (ARO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aromatase (ARO) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aromatase (ARO) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aromatase (ARO) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Aromatase

Target: Aromatase (CYP19A1) (Cytochrome P450, Family 19, Subfamily A, Polypeptide 1 (CYP19A1))

Alternative Name: Aromatase (CYP19A1 Products)

Synonyms: ARO ELISA Kit, ARO1 ELISA Kit, CPV1 ELISA Kit, CYAR ELISA Kit, CYP19 ELISA Kit, CYPXIX ELISA Kit, P-450AROM ELISA Kit, Aromatase ELISA Kit, Cyp19 ELISA Kit, Cyp19a ELISA Kit, p450arom ELISA Kit, P450 arom ELISA Kit, aro ELISA Kit, cyp19 ELISA Kit, cyp19a ELISA Kit, p450arom-A ELISA Kit, P450AROM ELISA Kit, Ar ELISA Kit, ArKO ELISA Kit, Int-5 ELISA Kit, Int5 ELISA Kit, CYP19P1 ELISA Kit, CYP19A1 ELISA Kit, CYP19a1 ELISA Kit, P450aromA ELISA Kit, ar1 ELISA Kit, cypXIX ELISA Kit, etID309862.18 ELISA Kit, zgc:136541 ELISA Kit, AroB ELISA Kit, CYP19a2 ELISA Kit, P450aromB ELISA Kit, cyp19b ELISA Kit, zgc:92614 ELISA Kit, cyp19a1 ELISA Kit, cytochrome P450 family 19 subfamily A member 1 ELISA Kit, cytochrome P450, family 19, subfamily a, polypeptide 1 ELISA Kit, cytochrome P450 family 19 subfamily A member 1 L homeolog ELISA Kit, aromatase ELISA Kit, cytochrome P450 aromatase ELISA Kit, cytochrome P450, family 19, subfamily A, polypeptide 1 ELISA Kit, cytochrome P450 19A1 ELISA Kit, cytochrome P450, family 17, subfamily A, polypeptide 1 ELISA Kit, cytochrome P450, family 19 ELISA Kit, cytochrome P450, family 19, subfamily A, polypeptide 1a ELISA Kit, cytochrome P450, family 19, subfamily A, polypeptide 1b ELISA Kit, cytochrome P450 19A1-like ELISA Kit, CYP19A1 ELISA Kit, Cyp19a1 ELISA Kit, cyp19a1.L ELISA Kit, SAV_2383 ELISA Kit, pfcyp19b-tstr ELISA Kit, CYP19 ELISA Kit, cyp19a1a ELISA Kit, cyp19a1b ELISA Kit, LOC101174651 ELISA Kit

Background:

Gene Name: Aromatase

Gene Aliases: CYP19A1, CYPXIX, ARO, ARO1, CPV1, CYAR, CYP19, P450AROM, P450-AROM, Estrogen synthase, Cytochrome P450,Family 19,Subfamily A,Polypeptide 1

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process, Carbohydrate Homeostasis