CLEC7A ELISA Kit
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- Target See all CLEC7A ELISA Kits
- CLEC7A (C-Type Lectin Domain Family 7, Member A (CLEC7A))
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Reactivity
- Mouse
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 0.156 ng/mL - 10 ng/mL
- Minimum Detection Limit
- 0.156 ng/mL
- Application
- ELISA
- Sample Type
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificity
- This assay has high sensitivity and excellent specificity for detection of C-Type Lectin Domain Family 7, Member A (CLEC7A). No significant cross-reactivity or interference between C-Type Lectin Domain Family 7, Member A (CLEC7A) and analogues was observed.
- Sensitivity
- 0.056 ng/mL
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- Comment
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Assay Time
- 3 h
- Plate
- Pre-coated
- Protocol
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to C-Type Lectin Domain Family 7, Member A (CLEC7A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to C-Type Lectin Domain Family 7, Member A (CLEC7A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C-Type Lectin Domain Family 7, Member A (CLEC7A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of C-Type Lectin Domain Family 7, Member A (CLEC7A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Assay Precision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level C-Type Lectin Domain Family 7, Member A (CLEC7A) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level C-Type Lectin Domain Family 7, Member A (CLEC7A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Handling Advice
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Storage
- 4 °C,-20 °C
- Storage Comment
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Expiry Date
- 4-8 months
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- Target See all CLEC7A ELISA Kits
- CLEC7A (C-Type Lectin Domain Family 7, Member A (CLEC7A))
- Alternative Name
- C-Type Lectin Domain Family 7, Member A (CLEC7A Products)
- Synonyms
- CLEC7A ELISA Kit, BGR ELISA Kit, Clecsf12 ELISA Kit, beta-GR ELISA Kit, CANDF4 ELISA Kit, CLECSF12 ELISA Kit, DECTIN1 ELISA Kit, Clec7b ELISA Kit, RGD1565140 ELISA Kit, C-type lectin domain containing 7A ELISA Kit, C-type lectin domain family 7, member a ELISA Kit, CLEC7A ELISA Kit, Clec7a ELISA Kit
- Background
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Gene Name: C-Type Lectin Domain Family 7, Member A
Gene Aliases: BGR, CLECSF1, BGR, CANDF4, CLECSF12, DECTIN1, Beta-glucan receptor, C-type lectin superfamily member 12, Dendritic cell-associated C-type lectin 1
- Pathways
- Activation of Innate immune Response
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