DICER1 ELISA Kit
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- Target See all DICER1 ELISA Kits
- DICER1 (Dicer 1, Ribonuclease Type III (DICER1))
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Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 0.312 ng/mL - 20 ng/mL
- Minimum Detection Limit
- 0.312 ng/mL
- Application
- ELISA
- Sample Type
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificity
- This assay has high sensitivity and excellent specificity for detection of Dicer 1, Ribonuclease Type III (DICER1). No significant cross-reactivity or interference between Dicer 1, Ribonuclease Type III (DICER1) and analogues was observed.
- Sensitivity
- 0.112 ng/mL
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- Comment
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Assay Time
- 3 h
- Plate
- Pre-coated
- Protocol
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dicer 1, Ribonuclease Type III (DICER1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dicer 1, Ribonuclease Type III (DICER1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dicer 1, Ribonuclease Type III (DICER1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dicer 1, Ribonuclease Type III (DICER1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Assay Precision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dicer 1, Ribonuclease Type III (DICER1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dicer 1, Ribonuclease Type III (DICER1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Handling Advice
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Storage
- 4 °C,-20 °C
- Storage Comment
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Expiry Date
- 4-8 months
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- Target See all DICER1 ELISA Kits
- DICER1 (Dicer 1, Ribonuclease Type III (DICER1))
- Alternative Name
- Dicer 1, Ribonuclease Type III (DICER1 Products)
- Synonyms
- dcr1 ELISA Kit, dicer ELISA Kit, herna ELISA Kit, DICER1 ELISA Kit, dicer1 ELISA Kit, 1110006F08Rik ELISA Kit, D12Ertd7e ELISA Kit, mKIAA0928 ELISA Kit, DCR1 ELISA Kit, Dicer ELISA Kit, HERNA ELISA Kit, MNG1 ELISA Kit, DICER ELISA Kit, fc39d11 ELISA Kit, fc74b05 ELISA Kit, wu:fc39d11 ELISA Kit, wu:fc74b05 ELISA Kit, dicer 1, ribonuclease III ELISA Kit, endoribonuclease Dicer ELISA Kit, dicer 1, ribonuclease III S homeolog ELISA Kit, dicer 1, ribonuclease type III ELISA Kit, dicer 1 ribonuclease III ELISA Kit, DICER1 ELISA Kit, CpipJ_CPIJ010534 ELISA Kit, dicer1 ELISA Kit, dicer1.S ELISA Kit, Dicer1 ELISA Kit
- Background
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Gene Name: Dicer 1, Ribonuclease Type III
Gene Aliases: Dicer, HERNA, Dicer 1,Double-Stranded RNA-Specific Endoribonuclease, Helicase with RNase motif, Helicase MOI
- Gene ID
- 23405
- UniProt
- Q9UPY3
- Pathways
- Regulatory RNA Pathways, Stem Cell Maintenance, Ribonucleoprotein Complex Subunit Organization
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