CD147 ELISA Kit

Catalog No. ABIN5654022

Product Details CD147 ELISA Kit

Target: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.25 pg/mL - 2000 pg/mL

Minimum Detection Limit: 31.25 pg/mL

Application: ELISA

Sample Type: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Extracellular Matrix Metalloproteinase Inducer (EMMPRIN). No significant cross-reactivity or interference between Extracellular Matrix Metalloproteinase Inducer (EMMPRIN) and analogues was observed.

Sensitivity: 12.7 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Extracellular Matrix Metalloproteinase Inducer (EMMPRIN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Extracellular Matrix Metalloproteinase Inducer (EMMPRIN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Extracellular Matrix Metalloproteinase Inducer (EMMPRIN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Extracellular Matrix Metalloproteinase Inducer (EMMPRIN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Extracellular Matrix Metalloproteinase Inducer (EMMPRIN) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Extracellular Matrix Metalloproteinase Inducer (EMMPRIN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for CD147

Target: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Alternative Name: Extracellular Matrix Metalloproteinase Inducer (BSG Products)

Synonyms: EMMPRIN ELISA Kit, RPE7 ELISA Kit, 5F7 ELISA Kit, CD147 ELISA Kit, M6 ELISA Kit, OK ELISA Kit, TCSF ELISA Kit, AI115436 ELISA Kit, AI325119 ELISA Kit, HT-7 ELISA Kit, 5A11 ELISA Kit, CE9 ELISA Kit, HT7 ELISA Kit, Ox47R ELISA Kit, basignin ELISA Kit, wu:fb26a05 ELISA Kit, wu:fo98e12 ELISA Kit, bsg ELISA Kit, prso03 ELISA Kit, basigin (Ok blood group) ELISA Kit, basigin ELISA Kit, basigin (Ok blood group) S homeolog ELISA Kit, BSG ELISA Kit, Bsg ELISA Kit, bsg ELISA Kit, bsg.S ELISA Kit

Background:

Gene Name: Extracellular Matrix Metalloproteinase Inducer

Gene Aliases: CD147, BSG, 5F7, M6, OK, TCSF, Basigin(Ok Blood Group), Collagenase stimulatory factor, OK blood group antigen, Tumor cell-derived collagenase stimulatory factor

Gene ID: 682

UniProt: P35613

Pathways: S100 Proteins