GADD45A ELISA Kit

Catalog No. ABIN5654826

Product Details GADD45A ELISA Kit

Target: GADD45A (Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Growth Arrest And DNA Damage Inducible Protein Alpha (GADD45a). No significant cross-reactivity or interference between Growth Arrest And DNA Damage Inducible Protein Alpha (GADD45a) and analogues was observed.

Sensitivity: 0.064 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Growth Arrest And DNA Damage Inducible Protein Alpha (GADD45a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Growth Arrest And DNA Damage Inducible Protein Alpha (GADD45a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Growth Arrest And DNA Damage Inducible Protein Alpha (GADD45a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Growth Arrest And DNA Damage Inducible Protein Alpha (GADD45a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Growth Arrest And DNA Damage Inducible Protein Alpha (GADD45a) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Growth Arrest And DNA Damage Inducible Protein Alpha (GADD45a) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for GADD45A

Target: GADD45A (Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A))

Alternative Name: Growth Arrest And DNA Damage Inducible Protein Alpha (GADD45A Products)

Synonyms: MGC53491 ELISA Kit, zgc:91795 ELISA Kit, gadd45a ELISA Kit, GADD45A ELISA Kit, ddit1 ELISA Kit, gadd45 ELISA Kit, GADD45 ELISA Kit, AA545191 ELISA Kit, Ddit1 ELISA Kit, DDIT1 ELISA Kit, Gadd45 ELISA Kit, growth arrest and DNA damage inducible alpha L homeolog ELISA Kit, growth arrest and DNA-damage-inducible, alpha, b ELISA Kit, growth arrest and DNA damage inducible alpha ELISA Kit, growth arrest and DNA damage-inducible protein 45 ELISA Kit, growth arrest and DNA-damage-inducible 45 alpha ELISA Kit, growth arrest and DNA-damage-inducible, alpha ELISA Kit, gadd45a.L ELISA Kit, gadd45ab ELISA Kit, GADD45A ELISA Kit, gadd45a ELISA Kit, GADD45 ELISA Kit, Gadd45a ELISA Kit

Background:

Gene Name: Growth Arrest And DNA Damage Inducible Protein Alpha

Gene Aliases: DDIT1, GADD45, DNA damage-inducible transcript 1 protein

UniProt: P48317

Pathways: p53 Signaling, Cell Division Cycle