IL12 CLIA Kit

Catalog No. ABIN5655751

Product Details IL12 CLIA Kit

Target: IL12 (Interleukin 12 (IL12))

Reactivity: Human

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 7.81 pg/mL - 500 pg/mL

Minimum Detection Limit: 7.81 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Interleukin 12 (IL12). No significant cross-reactivity or interference between Interleukin 12 (IL12) and analogues was observed.

Sensitivity: 0.28 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Interleukin 12 (IL12). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Interleukin 12 (IL12). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interleukin 12 (IL12) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 12 (IL12) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 12 (IL12) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: IL12 (Interleukin 12 (IL12))

Alternative Name: Interleukin 12 (IL12 Products)

Synonyms: CLMF CLIA Kit, CLMF2 CLIA Kit, IL-12B CLIA Kit, NKSF CLIA Kit, NKSF2 CLIA Kit, p40 CLIA Kit, Il-12b CLIA Kit, Il12p40 CLIA Kit, Il-12p40 CLIA Kit, Il12 CLIA Kit, IL-12p40 CLIA Kit, IL-12 CLIA Kit, LOC100217488 CLIA Kit, IL-12p35 CLIA Kit, Il-12a CLIA Kit, Ll12a CLIA Kit, p35 CLIA Kit, IL12p40 CLIA Kit, IL12B CLIA Kit, IL-12 p35 CLIA Kit, interleukin 12B CLIA Kit, interleukin 12A CLIA Kit, interleukin 12b CLIA Kit, interleukin 12a CLIA Kit, Interleukin 12 level CLIA Kit, IL12B CLIA Kit, IL12A CLIA Kit, Il12b CLIA Kit, Il12a CLIA Kit, IL12 CLIA Kit, il12a CLIA Kit

Background:

Gene Name: Interleukin 12

Pathways: JAK-STAT Signaling, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome