Macrophage Mannose Receptor 1 ELISA Kit
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- Target See all Macrophage Mannose Receptor 1 (MRC1) ELISA Kits
- Macrophage Mannose Receptor 1 (MRC1) (Mannose Receptor, C Type 1 (MRC1))
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Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 0.156 ng/mL - 10 ng/mL
- Minimum Detection Limit
- 0.156 ng/mL
- Application
- ELISA
- Sample Type
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificity
- This assay has high sensitivity and excellent specificity for detection of Mannose Receptor C Type 1 Like Protein 1 (MRC1). No significant cross-reactivity or interference between Mannose Receptor C Type 1 Like Protein 1 (MRC1) and analogues was observed.
- Sensitivity
- 0.062 ng/mL
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- Comment
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Assay Time
- 3 h
- Plate
- Pre-coated
- Protocol
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mannose Receptor C Type 1 Like Protein 1 (MRC1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mannose Receptor C Type 1 Like Protein 1 (MRC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mannose Receptor C Type 1 Like Protein 1 (MRC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mannose Receptor C Type 1 Like Protein 1 (MRC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Assay Precision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mannose Receptor C Type 1 Like Protein 1 (MRC1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mannose Receptor C Type 1 Like Protein 1 (MRC1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Handling Advice
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Storage
- 4 °C,-20 °C
- Storage Comment
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Expiry Date
- 4-8 months
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- Target See all Macrophage Mannose Receptor 1 (MRC1) ELISA Kits
- Macrophage Mannose Receptor 1 (MRC1) (Mannose Receptor, C Type 1 (MRC1))
- Alternative Name
- Mannose Receptor C Type 1 Like Protein 1 (MRC1 Products)
- Synonyms
- CD206 ELISA Kit, CLEC13D ELISA Kit, CLEC13DL ELISA Kit, MMR ELISA Kit, MRC1L1 ELISA Kit, bA541I19.1 ELISA Kit, AW259686 ELISA Kit, MR ELISA Kit, mrc1 ELISA Kit, MGC86525 ELISA Kit, MRC1 ELISA Kit, MRC1L-A ELISA Kit, mannose receptor C-type 1 ELISA Kit, mannose receptor, C type 1 ELISA Kit, mannose receptor C-type 1 L homeolog ELISA Kit, mediator of replication checkpoint 1 ELISA Kit, MRC1 ELISA Kit, Mrc1 ELISA Kit, mrc1.L ELISA Kit, mrc1 ELISA Kit
- Background
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Gene Name: Mannose Receptor C Type 1 Like Protein 1
Gene Aliases: CD206, CLEC13DL, MRC1L1, C-Type Lectin Domain Family 13 Member D-Like, Macrophage mannose receptor 1, Macrophage mannose receptor 1-like protein 1
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