Neuroserpin ELISA Kit
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- Target See all Neuroserpin (SERPINI1) ELISA Kits
- Neuroserpin (SERPINI1) (serpin Peptidase Inhibitor, Clade I (neuroserpin), Member 1 (SERPINI1))
- Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 0.781 ng/mL - 50 ng/mL
- Minimum Detection Limit
- 0.781 ng/mL
- Application
- ELISA
- Sample Type
- Plasma, Serum
- Analytical Method
- Quantitative
- Specificity
- This assay has high sensitivity and excellent specificity for detection of Neuroserpin (NSP). No significant cross-reactivity or interference between Neuroserpin (NSP) and analogues was observed.
- Sensitivity
- 0.34 ng/mL
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- Comment
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Assay Time
- 3 h
- Plate
- Pre-coated
- Protocol
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuroserpin (NSP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neuroserpin (NSP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neuroserpin (NSP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neuroserpin (NSP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Assay Precision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neuroserpin (NSP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neuroserpin (NSP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Handling Advice
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Storage
- 4 °C,-20 °C
- Storage Comment
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Expiry Date
- 4-8 months
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- Target See all Neuroserpin (SERPINI1) ELISA Kits
- Neuroserpin (SERPINI1) (serpin Peptidase Inhibitor, Clade I (neuroserpin), Member 1 (SERPINI1))
- Alternative Name
- Neuroserpin (SERPINI1 Products)
- Synonyms
- PI12 ELISA Kit, neuroserpin ELISA Kit, AI837402 ELISA Kit, Ns ELISA Kit, PI-12 ELISA Kit, Spi17 ELISA Kit, CG9453 ELISA Kit, Dmel\\CG9453 ELISA Kit, Serp2 ELISA Kit, Sp4 ELISA Kit, Spn4 ELISA Kit, Spn4A ELISA Kit, dSerp2 ELISA Kit, sp4 ELISA Kit, spn4 ELISA Kit, raPIT5a ELISA Kit, SERPINI1 ELISA Kit, pi12 ELISA Kit, serpini1l ELISA Kit, si:ch211-167c22.4 ELISA Kit, serpin family I member 1 ELISA Kit, serine (or cysteine) peptidase inhibitor, clade I, member 1 ELISA Kit, Serpin 42Da ELISA Kit, serpin peptidase inhibitor, clade I (neuroserpin), member 1 ELISA Kit, serpin family I member 1 L homeolog ELISA Kit, SERPINI1 ELISA Kit, Serpini1 ELISA Kit, Spn42Da ELISA Kit, serpini1 ELISA Kit, serpini1.L ELISA Kit
- Background
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Gene Name: Neuroserpin
Gene Aliases: SERPINI1, PI12, Serpin Peptidase Inhibitor Clade I Member 1, Peptidase inhibitor 12
- Gene ID
- 5274
- UniProt
- Q99574
- Pathways
- Regulation of Hormone Metabolic Process
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