POLD1 ELISA Kit

Catalog No. ABIN5657980

Product Details POLD1 ELISA Kit

Target: POLD1 (Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa (POLD1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Polymerase DNA Directed Delta 1 (POLd). No significant cross-reactivity or interference between Polymerase DNA Directed Delta 1 (POLd) and analogues was observed.

Sensitivity: 0.054 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Polymerase DNA Directed Delta 1 (POLd). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Polymerase DNA Directed Delta 1 (POLd). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Polymerase DNA Directed Delta 1 (POLd), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Polymerase DNA Directed Delta 1 (POLd) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Polymerase DNA Directed Delta 1 (POLd) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Polymerase DNA Directed Delta 1 (POLd) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for POLD1

Target: POLD1 (Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa (POLD1))

Alternative Name: Polymerase DNA Directed Delta 1 (POLD1 Products)

Synonyms: CDC2 ELISA Kit, CRCS10 ELISA Kit, POLD ELISA Kit, 5949 ELISA Kit, CAA61369 ELISA Kit, CG5949 ELISA Kit, DNA Pol Delta ELISA Kit, Dm Poldelta ELISA Kit, Dmel\\CG5949 ELISA Kit, Poldelta ELISA Kit, dnapol delta ELISA Kit, l(3)72Ac ELISA Kit, cdc2 ELISA Kit, pold ELISA Kit, 125kDa ELISA Kit, Pol delta ELISA Kit, fla ELISA Kit, im:7139126 ELISA Kit, zgc:152688 ELISA Kit, DNA polymerase delta 1, catalytic subunit ELISA Kit, DNA-polymerase-delta ELISA Kit, polymerase (DNA directed), delta 1, catalytic subunit ELISA Kit, DNA polymerase delta catalytic subunit ELISA Kit, DNA polymerase delta ELISA Kit, POLD1 ELISA Kit, DNApol-delta ELISA Kit, pold1 ELISA Kit, LOC100456571 ELISA Kit, Pold1 ELISA Kit

Background:

Gene Name: Polymerase DNA Directed Delta 1

Gene Aliases: CDC2, POLD1, DNA polymerase subunit delta p125

Gene ID: 5424

UniProt: P28340

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Chromatin Binding, Synthesis of DNA