RAB5 CLIA Kit

Catalog No. ABIN5658342

Product Details RAB5 CLIA Kit

Target: RAB5 (RAB5A) (RAB5A, Member RAS Oncogene Family (RAB5A))

Reactivity: Mouse

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 156.25 pg/mL - 10000 pg/mL

Minimum Detection Limit: 156.25 pg/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of RAB5A, Member RAS Oncogene Family (RAB5A). No significant cross-reactivity or interference between RAB5A, Member RAS Oncogene Family (RAB5A) and analogues was observed.

Sensitivity: 6.37 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to RAB5A, Member RAS Oncogene Family (RAB5A). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to RAB5A, Member RAS Oncogene Family (RAB5A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the RAB5A, Member RAS Oncogene Family (RAB5A) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level RAB5A, Member RAS Oncogene Family (RAB5A) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level RAB5A, Member RAS Oncogene Family (RAB5A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: RAB5 (RAB5A) (RAB5A, Member RAS Oncogene Family (RAB5A))

Alternative Name: RAB5A, Member RAS Oncogene Family (RAB5A Products)

Synonyms: RAB5 CLIA Kit, 2410015H04Rik CLIA Kit, AI663973 CLIA Kit, AU021172 CLIA Kit, nnyRab5a CLIA Kit, DDBDRAFT_0168594 CLIA Kit, DDBDRAFT_0229401 CLIA Kit, DDB_0168594 CLIA Kit, DDB_0229401 CLIA Kit, rab5 CLIA Kit, RAB5A CLIA Kit, Rab5a CLIA Kit, rab5al CLIA Kit, zgc:56644 CLIA Kit, fj38g08 CLIA Kit, hm:zehn1144 CLIA Kit, rab5a CLIA Kit, wu:fj38g08 CLIA Kit, RAB5A, member RAS oncogene family CLIA Kit, rab5A protein CLIA Kit, Rab5a, GTPase CLIA Kit, predicted protein CLIA Kit, Rab GTPase CLIA Kit, RAB5A, member RAS oncogene family L homeolog CLIA Kit, RAB5A, member RAS oncogene family, b CLIA Kit, RAB5A, member RAS oncogene family, a CLIA Kit, RAB5A CLIA Kit, Rab5a CLIA Kit, rab5A CLIA Kit, rab5a CLIA Kit, rab5a.L CLIA Kit, rab5ab CLIA Kit, rab5aa CLIA Kit

Background:

Gene Name: RAB5A, Member RAS Oncogene Family

Gene Aliases: RAB5

Pathways: Smooth Muscle Cell Migration, Regulation of long-term Neuronal Synaptic Plasticity