KIT CLIA Kit

Catalog No. ABIN5658954

Product Details KIT CLIA Kit

Target: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Reactivity: Human

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 468.75 pg/mL - 30000 pg/mL

Minimum Detection Limit: 468.75 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Stem Cell Factor Receptor (SCFR). No significant cross-reactivity or interference between Stem Cell Factor Receptor (SCFR) and analogues was observed.

Sensitivity: 15.3 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Stem Cell Factor Receptor (SCFR). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Stem Cell Factor Receptor (SCFR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Stem Cell Factor Receptor (SCFR) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Stem Cell Factor Receptor (SCFR) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Stem Cell Factor Receptor (SCFR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Alternative Name: Stem Cell Factor Receptor (KIT Products)

Synonyms: C-Kit CLIA Kit, CD117 CLIA Kit, PBT CLIA Kit, SCFR CLIA Kit, Bs CLIA Kit, Fdc CLIA Kit, Gsfsco1 CLIA Kit, Gsfsco5 CLIA Kit, Gsfsow3 CLIA Kit, SCO1 CLIA Kit, SCO5 CLIA Kit, SOW3 CLIA Kit, Ssm CLIA Kit, Tr-kit CLIA Kit, W CLIA Kit, c-KIT CLIA Kit, c-kit CLIA Kit, MGF CLIA Kit, KIT CLIA Kit, C-KIT CLIA Kit, kit CLIA Kit, receptor CLIA Kit, sparse CLIA Kit, Xkl-1 CLIA Kit, kit-a CLIA Kit, kit-b CLIA Kit, kl1-A CLIA Kit, krk1 CLIA Kit, pbt CLIA Kit, scfr CLIA Kit, KIT proto-oncogene receptor tyrosine kinase CLIA Kit, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog a CLIA Kit, KIT proto-oncogene receptor tyrosine kinase S homeolog CLIA Kit, KIT CLIA Kit, Kit CLIA Kit, kita CLIA Kit, kit CLIA Kit, kit.S CLIA Kit

Background:

Gene Name: Stem Cell Factor Receptor

Gene Aliases: CD117, KIT, C-Kit, PBT, p145 c-Kit, C-Kit Receptor, Tyrosine-Protein Kinase, V-Kit Hardy-Zuckerman 4 Feline Sarcoma Viral Oncogene Homolog, Piebald Trait

Gene ID: 3815

UniProt: P10721

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity