TDGF1 CLIA Kit
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- Target See all TDGF1 CLIA Kits
- TDGF1 (Teratocarcinoma-Derived Growth Factor 1 (TDGF1))
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Reactivity
- Human
- Detection Method
- Chemiluminescent
- Method Type
- Sandwich ELISA
- Detection Range
- 156.25 pg/mL - 10000 pg/mL
- Minimum Detection Limit
- 156.25 pg/mL
- Application
- ELISA
- Sample Type
- Tissue Homogenate
- Analytical Method
- Quantitative
- Specificity
- This assay has high sensitivity and excellent specificity for detection of Teratocarcinoma Derived Growth Factor 1 (TDGF1). No significant cross-reactivity or interference between Teratocarcinoma Derived Growth Factor 1 (TDGF1) and analogues was observed.
- Sensitivity
- 5.32 pg/mL
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- Comment
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Assay Time
- 2 - 3 h
- Plate
- Pre-coated
- Protocol
- The microplate provided in this kit has been pre-coated with an antibody specific to Teratocarcinoma Derived Growth Factor 1 (TDGF1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Teratocarcinoma Derived Growth Factor 1 (TDGF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Teratocarcinoma Derived Growth Factor 1 (TDGF1) level in the sample or standard.,
- Assay Precision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Teratocarcinoma Derived Growth Factor 1 (TDGF1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Teratocarcinoma Derived Growth Factor 1 (TDGF1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Handling Advice
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Storage
- 4 °C,-20 °C
- Storage Comment
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Expiry Date
- 4-8 months
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- Target See all TDGF1 CLIA Kits
- TDGF1 (Teratocarcinoma-Derived Growth Factor 1 (TDGF1))
- Alternative Name
- Teratocarcinoma Derived Growth Factor 1 (TDGF1 Products)
- Synonyms
- CR CLIA Kit, CRGF CLIA Kit, CRIPTO CLIA Kit, CR1 CLIA Kit, cripto CLIA Kit, cb85 CLIA Kit, tdgf1 CLIA Kit, wu:fe01d04 CLIA Kit, zgc:109829 CLIA Kit, Cripto-1 CLIA Kit, cr1 CLIA Kit, cr1a CLIA Kit, frl-1 CLIA Kit, frl1 CLIA Kit, xcr1 CLIA Kit, TDGF1 CLIA Kit, tdgf3 CLIA Kit, cripto-3 CLIA Kit, teratocarcinoma-derived growth factor 1 CLIA Kit, teratocarcinoma-derived growth factor 1, member 3 L homeolog CLIA Kit, teratocarcinoma-derived growth factor 1, member 1 CLIA Kit, TDGF1 CLIA Kit, Tdgf1 CLIA Kit, tdgf1 CLIA Kit, tdgf1.3.L CLIA Kit, tdgf1.1 CLIA Kit
- Background
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Gene Name: Teratocarcinoma Derived Growth Factor 1
Gene Aliases: CR, CRGF, CRIPTO, Cripto-1, Epidermal growth factor-like cripto protein CR1, Cripto-1 growth factor
- Pathways
- EGFR Signaling Pathway
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