VHL ELISA Kit

Catalog No. ABIN5659975

Product Details VHL ELISA Kit

Target: VHL (Von Hippel-Lindau Tumor Suppressor, E3 Ubiquitin Protein Ligase (VHL))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Von Hippel Lindau Tumor Suppressor (vHL). No significant cross-reactivity or interference between Von Hippel Lindau Tumor Suppressor (vHL) and analogues was observed.

Sensitivity: 0.113 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Von Hippel Lindau Tumor Suppressor (vHL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Von Hippel Lindau Tumor Suppressor (vHL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Von Hippel Lindau Tumor Suppressor (vHL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Von Hippel Lindau Tumor Suppressor (vHL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Von Hippel Lindau Tumor Suppressor (vHL) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Von Hippel Lindau Tumor Suppressor (vHL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for VHL

Target: VHL (Von Hippel-Lindau Tumor Suppressor, E3 Ubiquitin Protein Ligase (VHL))

Alternative Name: Von Hippel Lindau Tumor Suppressor (VHL Products)

Synonyms: HRCA1 ELISA Kit, RCA1 ELISA Kit, VHL1 ELISA Kit, pVHL ELISA Kit, BcDNA:RH61560 ELISA Kit, CG13221 ELISA Kit, DVhl ELISA Kit, Dmel\\CG13221 ELISA Kit, Dvhl ELISA Kit, VHL ELISA Kit, d-VHL ELISA Kit, d-vhl ELISA Kit, dVHL ELISA Kit, dmVHL ELISA Kit, vhl ELISA Kit, rca1 ELISA Kit, vhl1 ELISA Kit, hrca1 ELISA Kit, zgc:158722 ELISA Kit, Vhlh ELISA Kit, von Hippel-Lindau tumor suppressor ELISA Kit, von Hippel-Lindau ELISA Kit, von Hippel-Lindau tumor suppressor, E3 ubiquitin protein ligase ELISA Kit, von Hippel-Lindau disease tumor suppressor ELISA Kit, VHL ELISA Kit, Vhl ELISA Kit, vhl ELISA Kit, CpipJ_CPIJ009992 ELISA Kit

Background:

Gene Name: Von Hippel Lindau Tumor Suppressor

Gene Aliases: HRCA1, RCA1, VHL1, Von Hippel-Lindau Syndrome

Gene ID: 24874

UniProt: Q64259

Pathways: Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Ubiquitin Proteasome Pathway