WT1 CLIA Kit

Catalog No. ABIN5659991

Product Details WT1 CLIA Kit

Target: WT1 (Wilms Tumor 1 (WT1))

Reactivity: Human

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 156.25 pg/mL - 10000 pg/mL

Minimum Detection Limit: 156.25 pg/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Wilms Tumor Protein (WT1). No significant cross-reactivity or interference between Wilms Tumor Protein (WT1) and analogues was observed.

Sensitivity: 6.21 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Wilms Tumor Protein (WT1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Wilms Tumor Protein (WT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Wilms Tumor Protein (WT1) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wilms Tumor Protein (WT1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wilms Tumor Protein (WT1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: WT1 (Wilms Tumor 1 (WT1))

Alternative Name: Wilms Tumor Protein (WT1 Products)

Synonyms: AWT1 CLIA Kit, EWS-WT1 CLIA Kit, GUD CLIA Kit, NPHS4 CLIA Kit, WAGR CLIA Kit, WIT-2 CLIA Kit, WT33 CLIA Kit, CWT1 CLIA Kit, WT1 CLIA Kit, XWT1a CLIA Kit, wt-1 CLIA Kit, wt1 CLIA Kit, xWT1 CLIA Kit, WT-1 CLIA Kit, gud CLIA Kit, wagr CLIA Kit, wt33 CLIA Kit, xwt1 CLIA Kit, wit-2 CLIA Kit, si:ch211-160j6.3 CLIA Kit, zgc:152697 CLIA Kit, D630046I19Rik CLIA Kit, Wt-1 CLIA Kit, XWT1b CLIA Kit, XeWT1 CLIA Kit, Wilms tumor 1 CLIA Kit, Wilms tumor 1 S homeolog CLIA Kit, wilms tumor 1b CLIA Kit, Wilms tumor protein CLIA Kit, Wilms tumor 1 homolog CLIA Kit, Wilms tumor 1 L homeolog CLIA Kit, WT1 CLIA Kit, wt1.S CLIA Kit, wt1 CLIA Kit, wt1b CLIA Kit, Wt1 CLIA Kit, wt1.L CLIA Kit

Background:

Gene Name: Wilms Tumor Protein

Gene Aliases: WAGR, GUD, WIT-2, WT33

Gene ID: 7490

UniProt: P19544

Pathways: Tube Formation