WNT10A ELISA Kit
-
- Target See all WNT10A ELISA Kits
- WNT10A (Wingless-Type MMTV Integration Site Family, Member 10A (WNT10A))
-
Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 0.156 ng/mL - 10 ng/mL
- Minimum Detection Limit
- 0.156 ng/mL
- Application
- ELISA
- Sample Type
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificity
- This assay has high sensitivity and excellent specificity for detection of Wingless Type MMTV Integration Site Family, Member 10A (WNT10A). No significant cross-reactivity or interference between Wingless Type MMTV Integration Site Family, Member 10A (WNT10A) and analogues was observed.
- Sensitivity
- 0.061 ng/mL
- Featured
- Discover our best selling WNT10A ELISA Kit
- Top Product
- Discover our top product WNT10A ELISA Kit
-
-
- Comment
-
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Assay Time
- 3 h
- Plate
- Pre-coated
- Protocol
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wingless Type MMTV Integration Site Family, Member 10A (WNT10A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Wingless Type MMTV Integration Site Family, Member 10A (WNT10A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Wingless Type MMTV Integration Site Family, Member 10A (WNT10A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Wingless Type MMTV Integration Site Family, Member 10A (WNT10A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Assay Precision
-
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 10A (WNT10A) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 10A (WNT10A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
-
- Handling Advice
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Storage
- 4 °C,-20 °C
- Storage Comment
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Expiry Date
- 4-8 months
-
- Target See all WNT10A ELISA Kits
- WNT10A (Wingless-Type MMTV Integration Site Family, Member 10A (WNT10A))
- Alternative Name
- Wingless Type MMTV Integration Site Family, Member 10A (WNT10A Products)
- Synonyms
- WNT10A ELISA Kit, wnt10a/12 ELISA Kit, wnt10a ELISA Kit, OODD ELISA Kit, SSPS ELISA Kit, STHAG4 ELISA Kit, WNT-10A ELISA Kit, Wnt family member 10A ELISA Kit, protein Wnt-10a ELISA Kit, Wnt10a protein ELISA Kit, hypothetical protein ELISA Kit, WNT10A protein ELISA Kit, Wnt family member 10A L homeolog ELISA Kit, wingless-type MMTV integration site family, member 10A ELISA Kit, wingless-type MMTV integration site family, member 10a ELISA Kit, WNT10A ELISA Kit, LOC778792 ELISA Kit, CpipJ_CPIJ005839 ELISA Kit, Wnt10a ELISA Kit, wnt10a ELISA Kit, wnt10a.L ELISA Kit
- Background
-
Gene Name: Wingless Type MMTV Integration Site Family, Member 10A
- Pathways
- WNT Signaling
-