WNT5A CLIA Kit
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- Target See all WNT5A CLIA Kits
- WNT5A (Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A))
- Reactivity
- Human, Mouse, Rat
- Detection Method
- Chemiluminescent
- Method Type
- Sandwich ELISA
- Detection Range
- 156.25 pg/mL - 10000 pg/mL
- Minimum Detection Limit
- 156.25 pg/mL
- Application
- ELISA
- Sample Type
- Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificity
- This assay has high sensitivity and excellent specificity for detection of Wingless Type MMTV Integration Site Family, Member 5A (WNT5A). No significant cross-reactivity or interference between Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) and analogues was observed.
- Sensitivity
- 5.1 pg/mL
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- Comment
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Assay Time
- 2 - 3 h
- Plate
- Pre-coated
- Protocol
- The microplate provided in this kit has been pre-coated with an antibody specific to Wingless Type MMTV Integration Site Family, Member 5A (WNT5A). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Wingless Type MMTV Integration Site Family, Member 5A (WNT5A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) level in the sample or standard.,
- Assay Precision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Handling Advice
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Storage
- 4 °C,-20 °C
- Storage Comment
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Expiry Date
- 4-8 months
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- Target See all WNT5A CLIA Kits
- WNT5A (Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A))
- Alternative Name
- Wingless Type MMTV Integration Site Family, Member 5A (WNT5A Products)
- Synonyms
- hWNT5A CLIA Kit, wnt-5A CLIA Kit, wnt5a-B CLIA Kit, WNT5A CLIA Kit, 8030457G12Rik CLIA Kit, Wnt-5a CLIA Kit, Wnt family member 5A CLIA Kit, Wnt family member 5A S homeolog CLIA Kit, wingless-type MMTV integration site family, member 5a CLIA Kit, wingless-type MMTV integration site family, member 5A CLIA Kit, wingless-related MMTV integration site 5A CLIA Kit, Wnt signaling ligand CLIA Kit, WNT5A CLIA Kit, Wnt5a CLIA Kit, wnt5a.S CLIA Kit, wnt5a CLIA Kit, LOC578814 CLIA Kit, wnt5 CLIA Kit
- Background
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Gene Name: Wingless Type MMTV Integration Site Family, Member 5A
- Pathways
- WNT Signaling, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Cell Size, Tube Formation
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